Table of Contents: “…[et al.] -- Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo -- ES cell line establishment / Heidrun Kern and Branko Zevnik -- Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele -- Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. …”

Table of Contents: “…Overview on mouse mutagenesis / Ralf Kühn and Wolfgang Wurst -- Construction of gene-targeting vectors by recombineering / Song-Choon Lee, Wei Wang, and Pentao Liu -- Gene-trap vectors and mutagenesis / Silke De-Zolt [and others] -- Chromosome engineering in ES cells / Louise vander Weyden, Charles Shaw-Smith, and Allan Bradley -- Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts [and others] -- Generation of shRNA transgenic mice / Christiane Hitz [and others] -- Mutagenesis of mouse embryonic stem cells with ethylmethanesulfonate / Robert Munroe and John Schimenti -- Gene targeting in mouse embryonic stem cells / Lino Tessarollo [and others] -- Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo -- ES cell line establishment / Heidrun Kern and Branko Zevnik -- Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele -- Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. …”

Table of Contents: “…Overview: gene knockouts -- Isolation and maintenance of primate embryonic stem cells -- Gene targeting in ES cells -- Manipulating mouse embryonic stem cells -- Gene targeting in a centralized facility -- The loxP/CRE system and genome modification -- Creation and use of a Cre recombinase transgenic database -- Choice of mouse strains for gene targeting -- Isolation, microinjection, and transfer of mouse blastocysts -- Aggregatin chimeras: combining ES cells, diploid and tetraploid embryos -- How to study pathologic phenotypes of knockout mice -- Analysis of hematopoietic phenotypes in knockout mouse models -- Isolation of embryonic fibroblasts and their use in the in vitro characterization of gene function -- Influence of genetic background on knockout mouse phenotypes -- Lineage allocation during early embryogenesis: mapping of the neural primordia and application to the analysis of mouse mutants -- Generation of double-knockout, embryonic stem cells -- In vitro differentiation of embryonic stem cells and analysis of cellular phenotypes -- Embryonic stem cells in the study of hematopoiesis -- Interferon-inducible ES cell expression systems -- Transgenic studies in the mouse: improving the technology towards a conditional temporal and spatial approach -- In vivo libraries of large insert transgenic mice for genetic mapping -- Epigenetic effects on transgene expression -- Positional-candidate cloning of genes from mouse mutants -- Genetically engineered mice: husbandry and resources -- Embryo cryopreservation for transgenic mouse lines.…”

Table of Contents: “…How to Study Pathologic Phenotypes of Knockout Mice / Roderick T. Bronson -- 12. Analysis of Hematopoietic Phenotypes in Knockout Mouse Models / Ivan Bertoncello and Brenda Williams -- 13. …”

Table of Contents: “…The Transforming Growth Factor-[beta]1 Knockout Mouse: The Phenotype and Its Implications for TGF[beta]1 Function / Ashok B. …”

Table of Contents: “…Lentivirus transgenesis -- Lentivirus transgenesis -- Germline modification using mouse spermatogonial stem cells -- Embryonic in vivo electroporation in the mouse -- Current applications of transposons in mouse genetics -- Functional genomics in the mouse using the sleeping beauty transposon system -- The use of DNA transposons for cancer gene discovery in mice -- A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of creert2 -- A recombineering pipeline to make conditional targeting constructs -- Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria -- Genetic fate mapping using site-specific recombinases -- Mapping cell fate and function using recombinase-based intersectional strategies -- Genome-wide forward genetic screens in mouse es cells -- Gene trap mutagenesis in the mouse -- A wider context for gene trap mutagenesis -- Mouse mutagenesis with the chemical supermutagen enu -- Phenotype-driven mouse enu mutagenesis screens -- Using enu mutagenesis for phenotype-driven analysis of the mouse -- Exploration of self-renewal and pluripotency in es cells using rnai -- Transgenic rnai applications in the mouse -- Gene knockdown in the mouse through rnai -- In vivo analysis of gene knockdown in tetracycline-inducible shrna mice -- The power of reversibility regulating gene activities via tetracycline-controlled transcription -- Gene expression profiling of mouse oocytes and preimplantation embryos -- Interrogating the transcriptome of oocytes and preimplantation embryos -- Gene expression profiling of mouse embryos with microarrays -- Technological advances in mouse development studies.…”

Table of Contents: “…Saunders -- Using TARGATT technology to generate site-specific transgenic mice / Ruby Yanru Chen-Tsai -- Generating genetically engineered mice Using a Spermatogonial Stem Cell-Mediated Method / Takuya Sato and Takehiko Ogawa -- Chimeric Mouse Generation by ES Cell Blastocyst microinjection and uterine transfer / Yubin Du, Wen Xie, Fan Zhang, and Chengyu Liu -- Creating knockin alleles in mouse embryonic stem cells by CRISPR/Cas9-mediated homologous recombination without drug selection / Pinghu Liu, Yan Li, Jingqi Lei, and Lijin Dong -- Using CRISPR/Cas9 for gene knockout in immunodeficient NSG mice / Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, and Colin L. …”

Table of Contents: “…Fisch -- Transgenic mouse models and human psychiatric disease / Jonathan Flint -- Transgenic and knockout mouse models: the problem of language / Linda J. …”

Table of Contents: “…Transgenic and knockout mouse models : the problem of language / Linda J. …”

Table of Contents: “…Strategies for modulation of interleukin-1 in vivo: knockout and transgenics / F. F. Csaikl, U. M. Csaikl and S. …”

Table of Contents:

Table of Contents: “…Comprehensive and state-of-the-art, Transgenic Mouse Methods and Protocols, Second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice…”

Table of Contents: “…Gurumurthy, and Kay-Uwe Wagner -- 3. Generating Mouse Lines for Lineage Tracing and Knockout Studies / Petra Kraus, V. …”

Table of Contents: “…Saunders -- Gene Targeting in Mouse Embryonic Stem Cells / Jan van Deursen -- Conditional Knockout Mice / Ralf Kuhn, Frieder Schwenk -- Knockin Approaches / Anton J.M. …”

Table of Contents: “…Saunders -- Gene Targeting in Mouse Embryonic Stem Cells / Jan van Deursen -- Conditional Knockout Mice / Ralf Kuhn and Frieder Schwenk -- Knockin Approaches / Anton J. …”

Table of Contents: “…Subsequent sections explore more specialized subjects, considering optics, psychophysics, and the visual behaviors of mice; the organization of the adult mouse eye and central visual system; the development of the mouse eye (including comparisons to human development); the development and plasticity of retinal projections and visuotopic maps; mouse models for human eye disease (including glaucoma and cataracts); and the application of advanced genomic technologies (including gene therapy and genetic knockouts) to the mouse visual system. …”

Table of Contents: “…Festing -- Sophisticated mouse: protecting a precious reagent / Michael V. …”

Table of Contents: “…Focus: Diversity of the Mouse Central Auditory System / James F. Willott -- Ch. 18. …”

Table of Contents: “…Adjustment and rehabilitation problems of children, adolescents, and adults with Laron syndrome -- Laron syndrome patients with congenital IGF-I deficiency seem protected from malignant diseases -- Lifespan and mortality of patients with Laron syndrome -- IGF-I treatment of patients with Laron syndrome -- IGF-I stimulation of head growth in patients with Laron syndrome -- Effects of long-term administration of IGF-I on the adipose tissue and carbohydrate metabolism in children with Laron syndrome -- Comparison of the growth promoting response of IGF-I in children with Laron syndrome with that of hGH in children with isolated GH deficiency -- IGF-I treatment of adult patients with Laron syndrome -- Adverse effects encountered during IGF-I treatment of patients with Laron syndrome -- Summary of the clinical history: the Laron syndrome clock -- The Laron mouse -- Role of GH/IGF-I deficiency in aging -- Body composition, adipose tissue, and energy balance -- Metabolism and metabolic regulation -- Skeletal muscle -- Cardiac function in GHR-/-mice -- Bone -- GHR knockout and the CNS -- Cancer -- Reproduction -- Conclusions and future studies.…”

Table of Contents: “…1. Cytokine Knockouts in Inflammation / Pietro Ghezzi -- 2. The Use of Cytokine Knockouts to Study Host Defense Against Infection / Charles A. …”

Table of Contents: “…3.13 Additional Genes Known to Contribute to Gonadotrope Development4 CRE Mice for Targeting Gonadotropes; 5 Concluding Remarks; References; Chapter Two -- Mouse Models for the Study of Synthesis, Secretion, and Action of Pituitary Gonadotropins ; Abstract; 1 Introduction; 2 a-Glycoprotein Hormone Subunit; 2.1 Cga Transgenic Mice; 2.2 Cga Knockout Mice; 2.3 Other Cga Models; 3 LHß Subunit; 3.1 Lhb Transgenic Mice; 3.1.1 LHß-CTP Transgenic Mice; 3.1.2 HCGß Transgenic Mice; 3.1.3 Ubiquitin c-hCGß Mice; 3.1.4 MT-hCGß Transgenic Mice; 3.2 Lhb Knockout Mice; 4 FSHß Subunit; 4.1 Fshb Transgenic Mice.…”

Table of Contents: “…Adrenomedullin as an adipokine / Kazuhiro Takahashi -- Ch. 9. Adrenomedullin knockout mouse and transgenic mouse / Takayuki Shindo -- Ch. 10. …”

Table of Contents: “…Use of Confocal Microscopy Techniques in the Study of Transgenic and Knockout Mouse Genetics / James H. Resau, Eric A. Hudson and B. …”

Table of Contents: “…Introduction / Toshio Nishikimi -- Biochemistry of adrenomedullin / Kazuo Kitamura, Kenji Kangawa, and Tanenao Eto -- Adrenomedullin receptor and signal transduction / Takanobu Yoshimoto and Yukio Hirata -- Adrenomedullin gene / Toshihiko Ishimitsu, Kohju Tsukada, Junichi Minami, Hidehiko Ono, and Hiroaki Matsuoka -- Adrenomedullin expression and secretion / Naoto Minamino -- Biological action of adrenomedullin / Takeshi Horio and Fumiki Yoshihara -- Circulating adrenomedullin / Toshio Nishikimi -- Role of adrenomedullin in cardiovascular disease / Johji Kato and Tanenao Eto -- Adrenomedullin as an adipokine / Kazuhiro Takahashi -- Adrenomedullin knockout mouse and transgenic mouse / Takayuki Shindo -- Pleiotropic effect of adrenomedullin: lessons from pure adrenomedullin knockout mouse / Tatsuo Shimosawa, Katsuyuki Ando, and Toshiro Fujita -- Therapeutic application of adrenomedullin / Toshio Nishikimi, Noritoshi Nagaya, Kenji Kangawa, and Hiroaki Matsuoka -- Therapeutic potentials of adrenomedullin for heart failure and pulmonary hypertension / Noritoshi Nagaya, Toshio Nishikimi, and Kenji Kangawa.…”

Table of Contents: “…The Progesterone Receptor Knockout Mouse Model: New Insights into Progesterone Action In Vivo / John P. …”

Table of Contents: “…Bergner [and others] -- Knockout and mutant rats / M. Müller, J. Olivier and J. …”

Table of Contents: “…We therefore generated an adipocyte-specific SMRT knockout (adSMRT−/−) mouse to more clearly define SMRT's metabolic contributions. …”

Table of Contents: “…Genetics of galanin -- Glanin transgenic and knockout mouse models -- Galanin receptors and drugs -- Peripheral actions of galanin -- Galanin neuroendocrinology: sexual and feeding behavior -- Galanin in ascending systems -- Learning, memory, aging, and disease -- Galanin expression and nerve injury -- Poster papers.…”

Table of Contents: “…Conditional somatic mutagenesis in the mouse using site-specific recombinases -- Cre/loxP-mediated chromosome engineering of the mouse genome -- Tetracycline-controlled genetic switches -- Novel gene switches -- Improved embryonic stem cell technologies -- Gene trap mutagenesis -- RNA interference in mice -- Viral vectors: a wide range of choices and high levels of service -- Conditional mutagenesis by cell-permeable proteins: potential, limitations and prospects -- Analysis of mouse development with conditional mutagenesis -- Conditional mouse models of cancer -- Conditional mutagenesis reveals immunological functions of widely expressed genes: activation thresholds, homeostatic mechanisms and disease models -- Conditional transgenesis and recombination to study the molecular mechanisms of brain plasticity and memory -- A novel conditional knockout strategy applied to serotonin receptors -- Conditional mouse models for Friedreich Ataxia, a neurodegenerative disorder associating cardiomyopathy -- Animal models in cardiovascular diseases: new insights from conditional models -- Conditional animal models for the study of lipid metabolism and lipid disorders -- Conditional mouse models to study developmental and pathophysiological gene function in muscle -- Analysis of calcium channels by conditional mutagenesis -- Conditional mutagenesis of G-protein coupled receptors and G-proteins -- Contribution of targeted conditional somatic mutagenesis to deciphering retinoid X receptor functions and to generating mouse models of human diseases.…”

Table of Contents: “…Conditional somatic mutagenesis in the mouse using site-specific recombinases -- Cre/loxP-mediated chromosome engineering of the mouse genome -- Tetracycline-controlled genetic switches -- Novel gene switches -- Improved embryonic stem cell technologies -- Gene trap mutagenesis -- RNA interference in mice -- Viral vectors: a wide range of choices and high levels of service -- Conditional mutagenesis by cell-permeable proteins: potential, limitations and prospects -- Analysis of mouse development with conditional mutagenesis -- Conditional mouse models of cancer -- Conditional mutagenesis reveals immunological functions of widely expressed genes: activation thresholds, homeostatic mechanisms and disease models -- Conditional transgenesis and recombination to study the molecular mechanisms of brain plasticity and memory -- A novel conditional knockout strategy applied to serotonin receptors -- Conditional mouse models for Friedreich Ataxia, a neurodegenerative disorder associating cardiomyopathy -- Animal models in cardiovascular diseases: new insights from conditional models -- Conditional animal models for the study of lipid metabolism and lipid disorders -- Conditional mouse models to study developmental and pathophysiological gene function in muscle -- Analysis of calcium channels by conditional mutagenesis -- Conditional mutagenesis of G-protein coupled receptors and G-proteins -- Contribution of targeted conditional somatic mutagenesis to deciphering retinoid X receptor functions and to generating mouse models of human diseases.…”

Table of Contents: “…High-throughput life span assays in yeast and worms -- Genome-scale mouse knockout collection -- Collaborative cross and diversity outbred mice -- Expression QTLs -- Aging biomarkers -- Conclusions -- References -- 2 DNA damage and repair in aging -- Introduction -- Overview of the DNA damage response -- Endogenous DNA damage -- DNA damage induced by environmental exposures -- Linkage of DNA repair mechanisms to aging -- Nucleotide excision repair -- Base excision repair -- DNA double-strand break repair -- DNA damage theory of aging -- Aging disorders associated with DNA damage…”

Table of Contents: “…Huhtaniemi -- Understanding Molecular Mechanism of Toxicity and Carcinogenicity Using Gene Knockout and Transgenic Mouse Models / G. Elizondo and F. …”

Table of Contents: “…The In Vivo role of TNAP -- 8. Knockout mouse models -- 9. APs as physiological and disease markers -- 10. …”

Table of Contents: “…Introduction to ABC protein and gene organization -- Nomenclature -- Overview of human ABC gene subfamilies -- ABC genes and human genetic disease -- Mouse knockouts -- Multidrug resistance and cancer therapy -- Phylogenetic analysis of human ABC genes -- Mouse ABC genes -- Drosophila ABC genes -- ABCA genes -- ABCB genes -- ABCC genes -- ABCD genes -- ABCE genes -- ABCF genes -- ABCG genes.…”

Table of Contents: “…In recent years, the studies in urea transporter knockout mouse models suggest that urea transporters may be useful targets for drug discovery of selective inhibitors. …”

Table of Contents: “…Wang -- Large-scale mouse mutagenesis / E.J. Cartwright -- Dedicated mouse production and husbandry / L. …”

Table of Contents: “…Analysis of Serpin Secretion, Misfolding, and Surveillance in the Endoplasmic Reticulum -- Serpin-Enzyme Receptors: LDL Receptor-Related Protein 1 -- The Role of Autophagy in Alpha-1-Antitrypsin Deficiency -- Serpins and the Complement System -- Use of Mouse Models to Study Plasminogen Activator Inhibitor-1 -- Plasminogen Activator Inhibitor Type 2: Still an Enigmatic Serpin but a Model for Gene Regulation -- The SerpinB1 Knockout Mouse: A Model for Studying Neutrophil Protease Regulation in Homeostasis and Inflammation -- Investigating Maspin in Breast Cancer Progression Using Mouse Models -- Hsp47 as a collagen-specific molecular chaperone -- Assays for the Antiangiogenic and Neurotrophic Serpin Pigment Epithelium-Derived Factor -- The Drosophila Serpins: Multiple Functions in Immunity and Morphogenesis -- Modeling Serpin Conformational Diseases in Drosophila melanogaster -- Using Caenorhabditis elegans to Study Serpinopathies -- Using C. elegans to Identify the Protease Targets of Serpins In Vivo -- Viral Serpin Therapeutics: From Concept to Clinic -- man SCCA Serpins Inhibit Staphylococcal Cysteine Proteases by Forming Classic "Serpin-Like' Covalent Complexes -- Plants and the Study of Serpin Biology.…”

Table of Contents: “…Construction and evaluation of zinc finger nucleases / Hiroshi Ochiai and Takashi Yamamoto -- Current overview of TALEN construction systems / Tetsushi Sakuma and Takashi Yamamoto -- CRISPR/Cas9 / Izuho Hatada and Takuro Horii -- Computational prediction of CRISPR/Cas9 target sites reveals potential off-target risks in human and mouse / Qingbo Wang and Kumiko Ui-Tei -- Genome editing of mouse by cytoplasmic injection / Takuro Horii and Izuho Hatada -- Genome editing in mouse zygotes and embryonic stem cells by introducing SgRNA/Cas9 expressing plasmids / Taichi Noda, Asami Oji, and Masahito Ikawa -- Genome editing in mouse and rat by electroporation / Takehito Kaneko -- Generation of knock-in mouse by genome editing / Wataru Fujii -- Genome editing of rat / Takehito Kaneko -- Gene targeting in rabbits : single-step generation of knock-out rabbits by microinjection of CRISPR/Cas9 plasmids / Yoshihiro Kawano and Arata Honda -- Genome editing of pig / Masahito Watanabe and Hiroshi Nagashima -- Genome editing of monkey / Zhen Liu, Yijun Cai, and Qiang Sun -- Genome editing mediated by primordial germ cell in chicken / Jae Yong Han and Hong Jo Lee -- CRISPR/Cas9-mediated targeted knockin of exogenous reporter genes in zebrafish / Atsuo Kawahara -- Genome Editing of Medaka / Satoshi Ansai and Masato Kinoshita -- Simple protocol for loss-of-function analysis in Xenopus tropicalis founders using the CRISPR-Cas system / Yuto Sakane, Ken-ich T. …”

Table of Contents: “…Prohormone convertase 2 -- 3.1 Introduction to prohormone convertase 2 -- 3.1.1 Unique features -- 3.1.2 Evolution -- 3.1.3 Domain structure and functions -- 3.1.4 The PC2-binding protein 7B2 -- 3.1.5 Gene location -- 3.2 Distribution -- 3.2.1 Tissue distribution -- 3.2.2 Intracellular distribution -- 3.2.3 Cell lines -- 3.2.4 Development -- 3.3 Cell biology and maturation -- 3.3.1 ProPC2 maturation -- 3.3.2 7B2 and proPC2/PC2 targeting -- 3.4 Enzymatic characterization -- 3.4.1 General enzymatic properties -- 3.4.2 Contribution of various domains to enzymatic activity -- 3.4.3 Tissue analysis of PC2 activity -- 3.4.4 Substrate specificity -- 3.5 Regulation of expression and activity -- 3.5.1 Transcriptional and translational regulation -- 3.5.2 Endogenous inhibitors -- 3.5.3 Synthetic inhibitors and activators -- 3.5.4 Regulation of PC2 activity by its chaperone 7B2 -- 3.5.5 Regulation of 7B2 expression -- 3.6 Model systems, knockouts, and mutants -- 3.6.1 The PC2 knockout mouse -- 3.6.2 The 7B2 knockout mouse -- 3.6.3 Model systems -- 3.7 PC2 as a therapeutic target: disease relevance -- 3.7.1 Polymorphisms -- 3.7.2 Cancer --…”

Table of Contents: “…Describes the regulation of Sertoli cell and germ cell differentiation. Involving knockout and transgenic mouse models, the authors focus on male factor infertility that might be related to altered maturation of Sertoli cells, male factor infertility that might be due to incorrect histone-to-protamine exchange in haploid spermatids, and more.…”

Table of Contents: “…s PerspectiveInternational Harmonization of Laboratory Animals -- Rat Genetics and Toxicology -- A Phenotype-driven Approach to the Molecular and Functional Analysis of the Mouse Genome -- Evaluation of Targeted Mutations -- Defining Behavioral Phenotypes in Transgenic and Knockout Mice -- Defining Phenotype in Genetically Engineered Mice -- Development of the Mouse Model Dramatype for Human Clinical Benefit -- Concluding Remarks…”

Table of Contents: “…Cytokine Induction in NF-IL6 Family Knockout Mice; VII. Cytokine Induction in NF-KB Knockout Mice; VIII. …”

Table of Contents: “…Importantly, increased m6A levels were observed in mRNA isolated from the Alkbh5 knockout mouse organs compared to those from wild-type littermates. …”

Table of Contents: “…""3Department of Psychology, University of Houston""""126 Heyne Building, Houston, TX, US""; ""Abstract""; ""Introduction""; ""Autism Spectrum Disorders""; ""Small GTPases""; ""Rac, Cognition and Autism""; ""Regulation of Neuronal Morphology and Plasticity by Rac1""; ""Regulation of Learning and Memory by Rac1""; ""Fragile X Syndrome""; ""The Fmr1 Knockout Mice as an Animal Model for Fragile X Syndrome""; ""A Possible Link between Rac1 and Fragile X Syndrome""; ""Methodology""; ""Animals""; ""Generation of Rac1 Deficient Mice (Rac1 Mutant or Rac1 Knockout)""; ""Fmr1 Knockout Mouse""…”

Table of Contents: “…Partridge [and others] -- Phenotype changes of fut8 knockout mouse: Core fucosylation is crucial for the function of growth factor receptor(s) / X. …”

Table of Contents: “…Mutant, Transgenic, and Knockout Mouse Models -- 11. Cancer Models: Manipulating the Transforming Growth Factor-[beta] Pathway in Mice / John J. …”

Table of Contents: “…However, the failure of recent clinical trials that were based on successful preclinical studies using the Fmr1 knockout mouse model has forced the scientific community to revisit clinical trial design and identify objective outcome measures. …”

Table of Contents: “…Volume III guides readers through chapters on intercellular mitochondrial transfer, Cytoplasmic transfer methods, Ndufs4 knockout mouse, in vivo assessment of mitochondrial oxygen consumption, mitochondrial network analysis by imaging, mitochondrial respiration in platelets, Heterologous Inferential Analysis (HIA), bioenergetic profiling, integrative methods for studying cardiac energetics, and isolation and deep sequencing of mitochondrial DNA.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. …”

Table of Contents: “…Roep -- Building different mouse models for human MS / by Estelle Bettelli -- Autoimmunity and [beta] cell regeneration in mouse and human type 1 diabetes: the peace is not enough / by Vitaly Ablamunits ... …”

Table of Contents: “…Funderburgh -- Maintenance, transgene delivery, and pluripotency measurement of mouse embryonic stem cells / Tetsuya S. Tanaka -- Generation of a knockout mouse embryonic stem cell line using a paired CRISPR/Cas9 genome engineering tool / Rahel Wettstein, Maxime Bodak, and Constance Ciaudo -- Derivation of neural precursor cells from human embryonic stem cells for DNA methylomic analysis / Ivan Roubal, Sun Joo Park, and Yong Kim -- 26S and PA28-20S proteasome activity in cytosolic extracts from embryonic stem cells / Malin Hernebring -- Concise protocol for siRNA-mediated gene suppression in human embryonic stem cells / Peter F. …”

Table of Contents: “…Although CLC-3 is broadly expressed throughout the brain, the CLC-3 knockout mouse shows complete, selective postnatal neurodegeneration of the hippocampus, suggesting a crucial role for the channel in maintaining normal brain function. …”

Table of Contents: “…Gerton -- Establishment of a modified collagen-induced arthritis mouse model to investigate the anti-inflammatory activity of progranulin in inflammatory arthritis / Jian-Lu Wei and Chuan-Ju Liu -- Methods for studying the function of progranulin in atherosclerosis using both knockout mice models and in vitro studies / Tohru Ohama, Ryota Kawase, Yasushi Sakata, and Shizuya Yamashita -- Methods to investigate the roles of progranulin in angiogenesis using in vitro strategies and transgenic mouse models / Huishi Toh, Eugene Daniels, and Andrew Bateman.…”

Table of Contents: “…Li [et al.]. Transgenic Mouse Models and Knockout Mouse Models to Manipulate the Xenograft Response / I. …”

Table of Contents: “…Mattson, Weiming Fu and Peisu Zhang -- Ch. 8. The Telomerase Knockout Mouse / Maria A. Blasco -- Ch. 9. Telomerase in Brain Development and Neurodegenerative Disorders / Mark P. …”

Table of Contents: “…Cloning Full-Length Transcripts and Transcript Variants Using 5 and 3 RACE -- Monitoring Gene Expression: Quantitative Real-Time RT-PCR -- Microarray Technology: Basic Methodology and Application in Clinical Research for Biomarker Discovery in Vascular Diseases -- Northern Analysis of Gene Expression -- Laser Capture Microdissection for Analysis of Macrophage Gene Expression from Atherosclerotic Lesions -- Sequencing PCR-Amplified DNA in Lipoprotein and Cardiovascular Disease Research -- Introduction to Next-Generation Nucleic Acid Sequencing in Cardiovascular Disease Research -- Strategies for Designing Transgenic DNA Constructs -- Purification of Plasmid and BAC Transgenic DNA Constructs -- Pronuclear Microinjection and Oviduct Transfer Procedures for Transgenic Mouse Production -- Genotyping of Transgenic Animals by Real-Time Quantitative PCR with TaqMan Probes -- Generation of General and Tissue-Specific Gene Knockout Mouse Models -- Adeno-Associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism -- Modulation of Lipoprotein Metabolism by Antisense Technology: Preclinical Drug Discovery Methodology -- Chromatin Immunoprecipitation -- Measurement of Lecithin:Cholesterol Acyltransferase Activity with the Use of a Peptide-Proteoliposome Substrate -- Native-Native 2D Gel Electrophoresis for HDL Subpopulation Analysis -- Western Blots.…”

Table of Contents: “…Stellwag, and Baohong Zhang -- Genetic tools for self-organizing culture of mouse embryonic stem cells via small regulatory RNA-mediated technologies, CRISPR/Cas9, and inducible RNAi / Nozomu Takata, Eriko Sakakura, Tetsushi Sakuma, and Takashi Yamamoto -- CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells / Yinghua Wang, Yifu Ding, and Jinsong Li.…”

Table of Contents: “…Genome editing by targeted chromosomal mutagenesis -- Piggybac transposon-based insertional mutagenesis in mouse haploid embryonic stem cells -- Using phage integrases in a site-specific dual integrase cassette exchange strategy -- Therapeutic genome mutagenesis using synthetic donor DNA and triplex-forming molecules -- Genome engineering using adeno-associated virus (AAV) -- Engineering of customized meganucleases via in vitro compartmentalization and in cellulo optimization -- Efficient design and assembly of custom TALENs using the golden gate platform -- Ligation-independent cloning (LIC) assembly of TALEN genes -- Assembly and characterization of megatals for hyperspecific genome engineering applications -- Genome engineering using CRISPR-Cas9 system -- Donor plasmid design for codon and single base genome editing using zinc finger nucleases -- Endogenous gene tagging with fluorescent proteins -- Silencing long noncoding RNAs with genome-editing tools -- Gene editing using ssODNs with engineered endonucleases -- Genome editing in human pluripotent stem cells using site-specific nucleases -- Strategies to increase genome editing frequencies and to facilitate the identification of edited cells -- Using engineered endonucleases to create knockout and knockin zebrafish models -- Creating knockout and knockin rodents using engineered endonucleases via direct embryo injection -- Simple sperm preservation by freeze-drying for conserving animal strains.…”

Table of Contents: “…Contributions of Transgenic and Knockout Mice to Immunological Knowledge / Jose Moreno, Laura C. …”

Table of Contents: “…Animal Models of SSc Vasculopathy ; a) Fos-Related Antigen-2 (FRA-2) Transgenic Mouse Model ; b) Friend Leukemia Integration Factor-1 (Fli-1) Conditional Knockout Mimics SSc Vasculopathy.…”