Table of Contents: “…The physical basis of total internal reflection fluorescence (TIRF) microscopy and its cellular applications -- Two-photon excitation microscopy and its applications in neuroscience -- Live spheroid formation recorded with light sheet-based fluorescence microscopy -- Fluorescence microscopy-based RNA interference screening -- Fluorescence resonance energy transfer microscopy (FRET) -- Localizing protein-protein interactions in living cells using fluorescence lifetime imaging microscopy -- Analysis of biomolecular dynamics by FRAP and computer simulation -- Fluorescence correlation spectroscopy -- Homo-FRET imaging highlights the nanoscale organization of cell surface molecules -- Practical structured illumination microscopy -- 4Pi microscopy of the nuclear pore complex -- Application of STED microscopy to cell biology questions -- Three-dimensional photoactivated localization microscopy with genetically expressed probes -- Direct stochastic optical reconstruction microscopy (dSTORM) -- Optogenetics: Optical control of a photoactivatable Rac in living cells.…”

Table of Contents: “…Measurement of receptor-stimulated guanosine 5'-O-(γ-thio)triphosphate binding by G proteins / Thomas Wieland, Karl H Jakobs -- Receptor-stimulated hydrolysis of guanosine 5'-triphosphate in membrane preparations / Peter Gierschik, Thomas Bouillon, Karl H Jakobs -- Regulation of G-protein activation by mastoparans and other cationic peptides / Elliott M Ross, Tsutomu Higashuima -- Guanosine 5'-O-(γ-thio)triphosphate binding assay for solubilized G proteins / Donna J Carty, Ravi Iyengar -- Activation of cholera toxin by ADP-ribosylation factors: 20-kDa guanine nucleotide-binding proteins / Joel Moss, Randy S Haun, Su-Chen Tsai, Catherine F Welsh, Fang-Jen Scott Lee, S Russ Price, Martha Vaughan -- Pertussis toxin-catalyzed ADP-ribosylation of G proteins / Donna J Carty -- Synthesis and use of radioactive photoactivatable NAD+ derivatives as probes for G-protein structure / Richard R Vaillancourt, N Dhanasekaran, Arnold E Ruoho -- Photoaffinity guanosine 5'-triphosphate analogs as a tool for the study of GTP-binding proteins / Mark M Rasenick, Madhavi Talluri, William J Dunn III -- Preparation of activated α subunits of Gs and GiS: From erythrocyte to activated subunit / Lutz Birnbaumer, Dagoberto Grenet, Fernando Ribeiro-Neto, Juan Codina -- Purification and separation of closely related members of pertussis toxin-substrate G proteins / Toshiaki Katada, Kenji Kontani, Atsushi Inanobe, Ichiro Kobayashi, Yoshiharu Ohoka, Hiroshi Nishina, Katsunobu Takahashi -- Purification of transducin / Joëlle Bigay, Marc Chabre -- Expression of G-protein a subunits in Escherichia coli / Ethan Lee, Maurine E Linder, Alfred G Gilman -- Synthesis and applications of affinity matrix containing immobilized βγ subunits of G proteins / Iok-Hou Pang, Alan V Smrcka, Paul C Sternweis -- Purification of activated and heterotrimeric forms of Gq proteins / Jonathan L Blank, John H Exton -- Purification of Phospholipase C-activating G protein, G11, from Turkey erythrocytes / Gary L Waldo, José L Boyer, T Kendall Harden -- Purification of recombinant Gqα, G11α, and G16α from Sf9 cells / John R Hepler, Tohru Kozasa, Alfred G Gilman -- Expression and purification of G-protein α subunits using baculovirus expression system / Stephen G Graber, Robert A Figler, James C Garrison -- Analysis of G-protein α and βγ subunits by in vitro translation / Eva J Neer, Bradley M Denker, Thomas C Thomas, Carl J Schmidt -- Assays for studying functional properties of in vitro translated Gsα subunit / Yves Audigier -- Myristoylation of G-protein α subunits / Susanne M Mumby, Maurine E Linder -- Specificity and functional applications of antipeptide antisera which identify G-protein α subunits / Graeme Milligan -- Identification of receptor-activated G proteins: Selective immunoprecipitation of photolabeled G-protein α subunits / Karl-Ludwig Laugwitz, Karsten Spicher, Günter Schultz, Stefan Offermanns -- Identification of mutant forms of G-protein α subunits in human neoplasia by polymerase chain reaction-based techniques / John Lyons -- Detection of mutations and polymorphisms of Gsα subunit gene by denaturing gradient Gel electrophoresis / Pablo V Gejman, Lee S Weinstein -- Construction of mutant and chimeric G-protein α subunits / Sim Winitz, Marijane Russell, Gary L Johnson -- Design of degenerate oligonucleotide primers for cloning of G-protein a subunits / Thomas M Wilkie, Anna M Aragay, A John Watson, Melvin I Simon -- Microinjection of antisense oligonucleotides to assess G-protein subunit function / Christiane Kleuss, Günter Schultz, Burghardt Wittig -- Inactivation of G-protein genes: Double knockout in cell lines / Richard M Mortensen, J.G Seidman -- Targeted Inactivation of the G12α gene with replacement and insertion vectors: Analysis in a 96-well plate format / Uwe Rudolph, Allan Bradley, Lutz Birnbaumer -- G-Protein assays in Dictyostelium / B Ewa Snaar-Jagalska, Peter J.M Van Haastert -- Fluorescence assays for G-protein interactions / Richard A Cerione -- Specific peptide probes for G-protein interactions with receptors / Heidi E Hamm, Helen M Rarick -- Vaccinia virus systems for expression of Gα genes in S49 cells / Franklin Quan, Michael Forte -- Purification of Tβγ subunit of transducin / Joëlle Bigay, Marc Chabre -- Adenylyl cyclase assay for βγ subunits of G proteins / Jianqiang Chen, Donna J Carty, Ravi Iyengar -- Synthesis and use of biotinylated βγ complexes prepared from bovine brain G proteins / Jane Dingus, Michael D Wilcox, Russell Kohnken, John D Hildebrandt -- Design of oligonucleotide probes for molecular cloning of β and γ subunits / Christine Gallagher, Narasimhan Gautam -- Characterization of antibodies for various G-protein β and γ subunits / Alexey N Pronin, Narasimhan Gautam -- Preparation, characterization, and use of antibodies with specificity for G-protein y subunits / Janet D Robishaw, Eric A Balcueva -- Isoprenylation of γ subunits and G-protein effectors / Bernard K.…”

Table of Contents: “…Expression of GPCRs in Pichia pastoris for structural studies -- Conformational ensemble view of G protein-couples receptors and the effect of mutations and ligand binding -- Structural evolution of G-protein-coupled receptors: a sequence space approach -- Directed evolution of G-protein-coupled receptors for high functional expression and detergent stability -- The role of hydrophobic amino acids in the structure and function of the Rhodopsin family of G protein-coupled receptors -- Structure of [beta]-adrenergic receptors -- Advances in methods to characterize ligand-induced ionic lock and Rotamer toggle molecular switch in G protein-coupled receptors -- Crystallogenesis of adenosine A2A receptor-T4 lysozyme fusion protein: a practical route for the structure -- Probing GPCR structure: adenosine and P2Y nucleotide receptors -- Strategies for studying the ligand binding site of GPCRs: photoaffinity labeling of the VPAC1 receptor, a prototype of class B GPCRs -- Expression of mammalian G protein-coupled receptors in Caenorhabditis elegans -- Expression, purification, and structural analysis of intracellular C-termini from metabotropic glutamate receptors -- Unnatural amino acid mutagenesis of GPCRs using amber codon suppression and bioorthogonal labeling -- Mapping a ligand binding site using genetically encoded photoactivatable crosslinkers -- Alternative mRNA splicing of G protein-coupled receptors -- Functional residues essential for the activation of the C1 Cannabinoid receptor.…”

Table of Contents: “…Annotation This detailed volume encompasses new technological developments that specifically address questions related to adenosine 3',5'-monophosphate (cAMP) compartmentalisation, that probe relevant protein-protein interactions, that increase the spatial and temporal resolution of cAMP signal detection, and that can facilitate integration of the mounting complexity of the information that is becoming available on this signalling system.…”

Table of Contents: “…Chambers and Karin Sauer -- Probing protein-protein interactions with genetically encoded photoactivatable cross-linkers / Richard B. …”

Table of Contents: “…Signalling in Arbuscular Mycorrhiza: Facts and Hypotheses / Horst Vierheilig and Yves Piche -- 4. The Use of a Photoactivatable Kaempferol Analogue to Probe the Role of Flavonol 3-O-galactosyltransferase in Pollen Germination / Loverine P. …”

Table of Contents: “…In Transcription Factors: Methods and Protocols, experts in the field describe state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function. …”