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1. Investigation of Light-Regulated Protein–Protein Interactions Using Yeast Two-Hybrid Assays 2019

   https://ncsu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1LS8QwEA6iCOLBJ7q-iOBJCDZt0geIF1H2ILigHjzFJE1QhF2xLrI3_4P_0F_iTNIui3rx1oZuu_SbJjOT-b4h5MgXsoKFxjNZ-IKJKnXMQBTGwFeW1oAFWPEjmTMlhYVi925HMkzUHdetjXaqDAUlecqEgphnAeMrtOWbwXVnRSIrBHZkmmZakCqaYxAQ52dRZLHVLSzG8HkJGehecF-4LS87Faj2vJgKFf35D_5yS39tqYaV6nKFPHR8n1Cggm0RZiv1T4I6YpDEmFGC_Nf7WCXLSJCgyFwAUNbInBuuk8XY1HKyQZ5npDtGQzry9CqKlcSu966mA9SGeBp-fXy2RzTkJSPFoqGhioGePp7dY18hevs-Oj15PGMw0J8gv4yCSelJg4Ob5O7y4va8z9qWDswCJJzZUnBdmtTn3NdO1knqjXU-qfPEWUxu8lxnpa45JqtTX-fgANq0hJXcWBzKtgkVdV2CkwOONrp_zlSJ1pobmRmbaJeYHjnuQFFhx7ktc7XxtTRKVin8WiqpeNYjh9OL4cPC3RI9dKNxo2Ciw96aQlQ9shWhVS9RAURlHNwusBB4VAeOwic1qlOBBngUVwiPQngUwLPzn4t3yRK4ZlVM9uyR-bfXsdsn82gtB8HcvwEFjfPL

   Hiltbrunner, Andreas

   Methods In Molecular Biology (Clifton, N.J.), Vol. 2026, pp. 1 - 19.

   Yeast two-hybrid allows for investigation of interactions between two proteins in vivo. Yeast media can be supplemented with phycocyanobilin, allowing the formation of photoactive phytochromes, and... Read more

   Yeast two-hybrid allows for investigation of interactions between two proteins in vivo. Yeast media can be supplemented with phycocyanobilin, allowing the formation of photoactive phytochromes, and the investigation of their light-regulated interactions. Three reporter assays are described; the histidine growth selection assay for the HIS3 reporter, and the filter lift and quantitative ONPG assays for the LacZ reporter. Design considerations for yeast two- and three-hybrid experiments are also covered. Read less

   Book Chapter  |  Full Text Online

2. Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase... 2020

   https://ncsu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1La9wwEB6ahEIvfdNumi4qFHoS65UfsiGkpEuXLfTQQwulFyFZchzI2mntEPwb-qc7I9vbdQ699LIHawzWzqeZkTTzDcDbQsYZOpqCx7KQPMqE4wZ3YRxj5Tg3iIA8unOY02cTUi3MoN_RLHpbbeucjskXAoOFLKQ9-Pvrn5waR9EF69BF4wCO0Aingc_e-zIa4yQWvl8akXBytLyy521ELy0W2y3xgQrPqETdVfb80l3rvOeepqmTfdKk90XrR7AdZ-FTUKjxwX4u_sLzH3rSi5Hr8b9m_BgeDkErO-9R9gTuueop3O_bWHbP4Hdf7VsMx3-sLtjGfdaMLgWYJ4K4rBrWlrplxE5BdVmMzn_ZqkQ8dohQhrOkri1UasEurrq8RlyzpqvwpcYxSs6_YKflWUedhlh7W58uyjOOD8qOKs4Y7gB019DD5_Bt_fHrasOHJg88j9K45cYGIpd0-2ujLNLU8tymWoQYSBYIFC1DncrULk2indEyj5xIClsk2iYY2-AW-yWwyNoUwx4MvSkgdCYLtNZLE4cmD7QLzAzejbpV1z2Jh8LND6FAIQoUoUB5FKAkqV7R6qaZ66FIoa4c8WSpcwz4iD4njGdwMpHEVZlPh0eNqsEqNOqvOmfwZjdMb1KmW-XqGy9DqbLhEmVe9FjbfXMoPP1bNAM5QeFOgLjCpyPVZek5wyVZcxEd__uzXsED-i_6HLsTOGx_3bjXcEgInsOB_C7974c5HG1Wn9Y_5n6t_QEDMTO-

   Sun, Zhenjie; Sun, Yuhui; Li, Yumeng; Luan, Xiuli; Chen, Hui; Wu, Haiying...

   Molecular Medicine Reports, Vol. 21, Issue 3, pp. 1572 - 1580.

   Chlamydia trachomatis (C. trachomatis) is the leading cause of bacterial sexually transmitted diseases and infectious diseases that cause blindness. The pathophysiology of chlamydial infections is ... Read more

   Chlamydia trachomatis (C. trachomatis) is the leading cause of bacterial sexually transmitted diseases and infectious diseases that cause blindness. The pathophysiology of chlamydial infections is poorly understood, but secreted proteins have emerged as key virulence factors. C. trachomatis glycogen synthase (GlgA) is a chlamydial secretory protein, which localizes in the lumen of chlamydial inclusion bodies and the cytosol of host cells. In order to improve understanding of the roles of GlgA in chlamydial pathogenesis, four proteins that interact with GlgA, Homo sapiens CXXC finger protein 1, prohibitin (PHB), gelsolin‑like actin‑capping protein and apolipoprotein A‑I binding protein were identified using yeast two‑hybrid assays. The functions of these proteins are complex, and preliminary results suggested that PHB interacts with GlgA. However, further studies are required to determine the specific interactions of these proteins with GlgA. The findings of the present study may provide a direction and foundation for future studies focusing on the mechanism of GlgA in C. trachomatis infection. Read less

   Journal Article  |  Full Text Online

3. Identification of Toxocara canis Antigen-Interacting Partners by Yeast Two-Hybrid Assay and a... 2021

   https://ncsu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NjtMwEB6xi5C4IP4kAktlJE5I3qa2U8fSaqWloiqHlXooB7hEjuNsi2iyalpBb7wDr8CT8STMOGlF4MbVzo-TGXu-sWe-AXhd6sSgoSl5okvNlRGe5-iFccTKictRA5z6azMHkkMuTAjad_nqvPqyPq9WyxBbebt2w0Oc2HB-PUGjTvWxhidwoqX6w0X_3EGgdKTbE0yJLj2FVi9rFEdDRDbozhBTqCRiLjWSPXMUWPt7ULMfKNnSSAXLM30IDzrIyK7aoT2CO756DPfaIpL7J_CzzbUtu803VpdsUX9DE7WxDP_bqmFX2I0D42H3jxIZqhs2xy-tEPqxfM8ulpcfqYQPW3ytL4bLS44Nsz2lcjGUnt1TG7NVwSyb77aBKZxde0oZXjXr8L6lbzyb1c321_cf-GRLR9Ls-DpU7KfwYfpuMZnxrvYCd0roLSfiN1soIuiTiZa5icc2dg7xSFIIh5Ne4OpU6FIJawr0-byUo1DusxBKu7GRz4CpokgRjSAiJpzm8RnW2lGeyNzF1sd5BG8O_z67bbk1MvRJSGbZPzKL4C1J53gh0WKHhnpzk3XKkdEpH67XpdGIWpQ3xuKCpkuJNtilplQRnB1km3VztckQ9CXEqx_HEbw6duMso6MTW_l6F64Zx8KMRRqB7ulEb0D9HlTfwNfdqevz_77zBdwXFEoTay7SMzjdbnb-JZxSkscA7s4m76efBhS0Oh-EqfAbmQgQSA

   Długosz, Ewa; Milewska, Małgorzata; Bąska, Piotr

   Pathogens (Basel), Vol. 10, Issue 8, p. 949.

   Toxocara canis is a zoonotic roundworm that infects humans and dogs all over the world. Upon infection, larvae migrate to various tissues leading to different clinical syndromes. The host–parasite ... Read more

   Toxocara canis is a zoonotic roundworm that infects humans and dogs all over the world. Upon infection, larvae migrate to various tissues leading to different clinical syndromes. The host–parasite interactions underlying the process of infection remain poorly understood. Here, we describe the application of a yeast two-hybrid assay to screen a human cDNA library and analyse the interactome of T. canis larval molecules. Our data identifies 16 human proteins that putatively interact with the parasite. These molecules were associated with major biological processes, such as protein processing, transport, cellular component organisation, immune response and cell signalling. Some of these identified interactions are associated with the development of a Th2 response, neutrophil activity and signalling in immune cells. Other interactions may be linked to neurodegenerative processes observed during neurotoxocariasis, and some are associated with lung pathology found in infected hosts. Our results should open new areas of research and provide further data to enable a better understanding of this complex and underestimated disease. Read less

   Journal Article  |  Full Text Online

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