What is the role of microbial biotechnology and genetic engineering in medicine?
Academic Journal
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Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101588314 Publication Model: Print Cited Medium: Internet ISSN: 2045-8827 (Electronic) Linking ISSN: 20458827 NLM ISO Abbreviation: Microbiologyopen Subsets: MEDLINE
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Santos-Beneit F
Academic Journal
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Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101588314 Publication Model: Print Cited Medium: Internet ISSN: 2045-8827 (Electronic) Linking ISSN: 20458827 NLM ISO Abbreviation: Microbiologyopen Subsets: MEDLINE
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Microbial products are essential for developing various therapeutic agents, including ...
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What is the role of microbial biotechnology and genetic engineering in medicine?
Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101588314 Publication Model: Print Cited Medium: Internet ISSN: 2045-8827 (Electronic) Linking ISSN: 20458827 NLM ISO Abbreviation: Microbiologyopen Subsets: MEDLINE
Microbial products are essential for developing various therapeutic agents, including antibiotics, anticancer drugs, vaccines, and therapeutic enzymes. Genetic engineering techniques, functional genomics, and synthetic biology unlock previously uncharacterized natural products. This review highlights major advances in microbial biotechnology, focusing on gene-based technologies for medical applications.
(© 2024 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
(© 2024 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
Subject terms:
Genetic Techniques - Genomics - Synthetic Biology - Biotechnology methods - Genetic EngineeringContent provider:
MEDLINE
Full Text from ProQuest
Biotechnology and genetic engineering / Kathy Wilson Peacock ; foreword by Charles Hagedorn.
Book
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2010
Book
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2010
Available at LC Collection (TP248.215 .P43 2010)
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RNA-based controllers for engineering gene and cell therapies.
Academic Journal
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Publisher: Elsevier Country of Publication: England NLM ID: 9100492 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0429 (Electronic) Linking ISSN: 09581669 NLM ISO Abbreviation: Curr Opin Biotechnol Subsets: MEDLINE
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Takahashi K;Galloway KE
Academic Journal
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Publisher: Elsevier Country of Publication: England NLM ID: 9100492 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0429 (Electronic) Linking ISSN: 09581669 NLM ISO Abbreviation: Curr Opin Biotechnol Subsets: MEDLINE
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Engineered RNA-based genetic controllers provide compact, tunable, post-transcriptiona...
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RNA-based controllers for engineering gene and cell therapies.
Publisher: Elsevier Country of Publication: England NLM ID: 9100492 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0429 (Electronic) Linking ISSN: 09581669 NLM ISO Abbreviation: Curr Opin Biotechnol Subsets: MEDLINE
Engineered RNA-based genetic controllers provide compact, tunable, post-transcriptional gene regulation. As RNA devices are generally small, these devices are portable to DNA and RNA viral vectors. RNA tools have recently expanded to allow reading and editing of endogenous RNAs for profiling and programming of transcriptional states. With their expanded capabilities and highly compact, modular, and programmable nature, RNA-based controllers will support greater safety, efficacy, and performance in gene and cell-based therapies. In this review, we highlight RNA-based controllers and their potential as user-guided and autonomous systems for control of gene and cell-based therapies.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Subject terms:
Gene Expression Regulation - Cell- and Tissue-Based Therapy - Genetic Vectors - Gene Editing - CRISPR-Cas Systems - RNA genetics - Genetic EngineeringContent provider:
MEDLINE
The hope, hype & reality of genetic engineering : remarkable stories from agriculture, industry, medicine, and the environment / John C. Avise.
Book
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2004
Book
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2004
Available at LC Collection (QH442 .A98 2004)
Plastid engineering using episomal DNA.
Academic Journal
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Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE
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Occhialini A;Lenaghan SC
Academic Journal
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Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE
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Key Message: Novel episomal systems have the potential to accelerate plastid genetic e...
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Plastid engineering using episomal DNA.
Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE
Key Message: Novel episomal systems have the potential to accelerate plastid genetic engineering for application in plant synthetic biology. Plastids represent valuable subcellular compartments for genetic engineering of plants with intrinsic advantages to engineering the nucleus. The ability to perform site-specific transgene integration by homologous recombination (HR), coordination of transgene expression in operons, and high production of heterologous proteins, all make plastids an attractive target for synthetic biology. Typically, plastid engineering is performed by homologous recombination; however, episomal-replicating vectors have the potential to accelerate the design/build/test cycles for plastid engineering . By accelerating the timeline from design to validation, it will be possible to generate translational breakthroughs in fields ranging from agriculture to biopharmaceuticals. Episomal-based plastid engineering will allow precise single step metabolic engineering in plants enabling the installation of complex synthetic circuits with the ambitious goal of reaching similar efficiency and flexibility of to the state-of-the-art genetic engineering of prokaryotic systems. The prospect to design novel episomal systems for production of transplastomic marker-free plants will also improve biosafety for eventual release in agriculture.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Subject terms:
Plants genetics - Transgenes genetics - Metabolic Engineering - DNA metabolism - Plants, Genetically Modified genetics - Transformation, Genetic - Genetic Engineering methods - Plastids genetics - Plastids metabolismContent provider:
MEDLINE
Genetic engineering : opposing viewpoints / James D. Torr, book editor.
Book
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2001
Book
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2001
Available at LC Collection (QH442 .G4432 2001)
Zhu R;Zhang J;Wang L;Zhang Y;Zhao Y;Han Y;Sun J;Zhang X;Dou Y;Yao H;Yan W;L...
Academic Journal
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Publisher: Cell Press Country of Publication: United States NLM ID: 101656080 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2405-4720 (Electronic) Linking ISSN: 24054712 NLM ISO Abbreviation: Cell Syst Subsets: MEDLINE
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Functionalizing materials with biomacromolecules such as enzymes has broad application...
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Publisher: Cell Press Country of Publication: United States NLM ID: 101656080 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2405-4720 (Electronic) Linking ISSN: 24054712 NLM ISO Abbreviation: Cell Syst Subsets: MEDLINE
Functionalizing materials with biomacromolecules such as enzymes has broad applications in biotechnology and biomedicine. Here, we introduce a grafting method mediated by living cells to functionalize materials. We use polymeric scaffolds to trap engineered bacteria and micron-sized particles with chemical groups serving as active sites for grafting. The bacteria synthesize the desired protein for grafting and autonomously lyse to release it. The released functional moieties are locally grafted onto the active sites, generating the materials engineered by living grafting (MELGs). MELGs are resilient to perturbations because of both the bonding and the regeneration of functional domains synthesized by living cells. The programmability of the bacteria enables us to fabricate MELGs that can respond to external input, decompose a pollutant, reconstitute synthetic pathways for natural product synthesis, and purify mismatched DNA. Our work establishes a bacteria-assisted grafting strategy to functionalize materials with a broad range of biological activities in an integrated, flexible, and modular manner. A record of this paper's transparent peer review process is included in the supplemental information.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Subject terms:
Proteins - Synthetic Biology - Bacteria genetics - Biotechnology - Genetic EngineeringContent provider:
MEDLINE
Biotechnology and genetic engineering / Lisa Yount.
Book
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2000
Book
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2000
Available at Dewey Collection (303.483 Y883b)
R we there yet? Advances in cloning resistance genes for engineering immunity in crop plants.
Academic Journal
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Publisher: Current Biology Ltd Country of Publication: England NLM ID: 100883395 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0356 (Electronic) Linking ISSN: 13695266 NLM ISO Abbreviation: Curr Opin Plant Biol Subsets: MEDLINE
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Chen R;Gajendiran K;Wulff BBH
Academic Journal
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Publisher: Current Biology Ltd Country of Publication: England NLM ID: 100883395 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0356 (Electronic) Linking ISSN: 13695266 NLM ISO Abbreviation: Curr Opin Plant Biol Subsets: MEDLINE
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Over the past three decades, significant progress has been made in the field of resist...
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R we there yet? Advances in cloning resistance genes for engineering immunity in crop plants.
Publisher: Current Biology Ltd Country of Publication: England NLM ID: 100883395 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0356 (Electronic) Linking ISSN: 13695266 NLM ISO Abbreviation: Curr Opin Plant Biol Subsets: MEDLINE
Over the past three decades, significant progress has been made in the field of resistance (R) gene cloning. Advances in recombinant DNA technology, genome sequencing, bioinformatics, plant transformation and plant husbandry have facilitated the transition from cloning R genes in model species to crop plants and their wild relatives. To date, researchers have isolated more than 450 R genes that play important roles in plant immunity. The molecular and biochemical mechanisms by which intracellular immune receptors are activated and initiate defense responses are now well understood. These advances present exciting opportunities for engineering disease-resistant crop plants that are protected by genetics rather than pesticides.
Competing Interests: Declaration of competing interest The authors have no competing interests to declare.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Competing Interests: Declaration of competing interest The authors have no competing interests to declare.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Subject terms:
Cloning, Molecular - Plant Immunity genetics - Genes, Plant - Disease Resistance genetics - Plant Diseases genetics - Plants genetics - Genetic EngineeringContent provider:
MEDLINE
Genetic engineering : a reference handbook / Harry Le Vine III.
Book
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1999
Book
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1999
Available at LC Collection (TP248.6 .L4 1999)
Using design of experiments to guide genetic optimization of engineered metabolic pathways.
Academic Journal
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Publisher: Oxford University Press Country of Publication: Germany NLM ID: 9705544 Publication Model: Print Cited Medium: Internet ISSN: 1476-5535 (Electronic) Linking ISSN: 13675435 NLM ISO Abbreviation: J Ind Microbiol Biotechnol Subsets: MEDLINE
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Moon S;Saboe A;Smanski MJ
Academic Journal
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Publisher: Oxford University Press Country of Publication: Germany NLM ID: 9705544 Publication Model: Print Cited Medium: Internet ISSN: 1476-5535 (Electronic) Linking ISSN: 13675435 NLM ISO Abbreviation: J Ind Microbiol Biotechnol Subsets: MEDLINE
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Design of experiments (DoE) is a term used to describe the application of statistical ...
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Using design of experiments to guide genetic optimization of engineered metabolic pathways.
Publisher: Oxford University Press Country of Publication: Germany NLM ID: 9705544 Publication Model: Print Cited Medium: Internet ISSN: 1476-5535 (Electronic) Linking ISSN: 13675435 NLM ISO Abbreviation: J Ind Microbiol Biotechnol Subsets: MEDLINE
Design of experiments (DoE) is a term used to describe the application of statistical approaches to interrogate the impact of many variables on the performance of a multivariate system. It is commonly used for process optimization in fields such as chemical engineering and material science. Recent advances in the ability to quantitatively control the expression of genes in biological systems open up the possibility to apply DoE for genetic optimization. In this review targeted to genetic and metabolic engineers, we introduce several approaches in DoE at a high level and describe instances wherein these were applied to interrogate or optimize engineered genetic systems. We discuss the challenges of applying DoE and propose strategies to mitigate these challenges.
One-Sentence Summary: This is a review of literature related to applying Design of Experiments forgenetic optimization.
(© The Author(s) 2024. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology.)
One-Sentence Summary: This is a review of literature related to applying Design of Experiments for
(© The Author(s) 2024. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology.)
Subject terms:
Metabolic Engineering - Metabolic Networks and Pathways genetics - Genetic EngineeringContent provider:
MEDLINE
Genetic engineering of plants for crop improvement / authors, Rup Lal, Sukanya Lal.
Book
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1993
Book
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1993
Available at LC Collection (SB123.57 .R875 1993)
Hoffmann SA;Cai Y
Academic Journal
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Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
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Synthetic biology holds immense promise to tackle key problems in resource use, enviro...
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Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
Synthetic biology holds immense promise to tackle key problems in resource use, environmental remediation, and human health care. However, comprehensive safety measures are lacking to employ engineered microorganisms in open-environment applications. Genetically encoded biocontainment systems may solve this issue. Here, we describe such a system based on conditional stability of essential proteins. We used a destabilizing domain degron stabilized by estradiol addition (ERdd). We ERdd-tagged 775 essential genes and screened for strains with estradiol dependent growth. Three genes, SPC110, DIS3 and RRP46, were found to be particularly suitable targets. Respective strains showed no growth defect in the presence of estradiol and strong growth inhibition in its absence. SPC110-ERdd offered the most stringent containment, with an escape frequency of <5×10 -7 . Removal of its C-terminal domain decreased the escape frequency further to <10 -8 . Being based on conditional protein stability, the presented approach is mechanistically orthogonal to previously reported genetic biocontainment systems.
(© 2024. The Author(s).)
(© 2024. The Author(s).)
Subject terms:
Humans - Organisms, Genetically Modified genetics - Containment of Biohazards - Synthetic Biology - Estradiol metabolism - Saccharomyces cerevisiae genetics - Genetic EngineeringContent provider:
MEDLINE
Genetic engineering : opposing viewpoints / William Dudley, book editor.
Book
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1990
Book
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1990
Available at Dewey Collection (179.1 G286)
Outlook on genome editing application to cattle.
Academic Journal
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Publisher: Korean Society of Veterinary Science Country of Publication: Korea (South) NLM ID: 100964185 Publication Model: Print Cited Medium: Internet ISSN: 1976-555X (Electronic) Linking ISSN: 1229845X NLM ISO Abbreviation: J Vet Sci Subsets: MEDLINE
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Gim GM;Jang G
Academic Journal
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Publisher: Korean Society of Veterinary Science Country of Publication: Korea (South) NLM ID: 100964185 Publication Model: Print Cited Medium: Internet ISSN: 1976-555X (Electronic) Linking ISSN: 1229845X NLM ISO Abbreviation: J Vet Sci Subsets: MEDLINE
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In livestock industry, there is growing interest in methods to increase the production...
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Outlook on genome editing application to cattle.
Publisher: Korean Society of Veterinary Science Country of Publication: Korea (South) NLM ID: 100964185 Publication Model: Print Cited Medium: Internet ISSN: 1976-555X (Electronic) Linking ISSN: 1229845X NLM ISO Abbreviation: J Vet Sci Subsets: MEDLINE
In livestock industry, there is growing interest in methods to increase the production efficiency of livestock to address food shortages, given the increasing global population. With the advancements in gene engineering technology, it is a valuable tool and has been intensively utilized in research specifically focused on human disease. In historically, this technology has been used with livestock to create human disease models or to produce recombinant proteins from their byproducts. However, in recent years, utilizing gene editing technology, cattle with identified genes related to productivity can be edited, thereby enhancing productivity in response to climate change or specific disease instead of producing recombinant proteins. Furthermore, with the advancement in the efficiency of gene editing, it has become possible to edit multiple genes simultaneously. This cattle breed improvement has been achieved by discovering the genes through the comprehensive analysis of the entire genome of cattle. The cattle industry has been able to address gene bottlenecks that were previously impossible through conventional breeding systems. This review concludes that gene editing is necessary to expand the cattle industry, improving productivity in the future. Additionally, the enhancement of cattle through gene editing is expected to contribute to addressing environmental challenges associated with the cattle industry. Further research and development in gene editing, coupled with genomic analysis technologies, will significantly contribute to solving issues that conventional breeding systems have not been able to address.
Competing Interests: The authors declare no conflicts of interest.
(© 2024 The Korean Society of Veterinary Science.)
Competing Interests: The authors declare no conflicts of interest.
(© 2024 The Korean Society of Veterinary Science.)
Subject terms:
Animals - Cattle genetics - Humans - Breeding - Genome - Livestock genetics - Recombinant Proteins - Gene Editing veterinary - Genetic Engineering methods - Genetic Engineering veterinaryContent provider:
MEDLINE
Genetic engineering in the plant sciences / edited by Nickolas J. Panopoulos.
Book
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1981
Book
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1981
Available at Dewey Collection (581.87322 G286)
The current situations and limitations of genetic engineering in cyanobacteria: a mini review.
Academic Journal
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Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE
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Cheng J;Zhang K;Hou Y
Academic Journal
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Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE
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Cyanobacteria are an ancient group of photoautotrophic prokaryotes, and play an essent...
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The current situations and limitations of genetic engineering in cyanobacteria: a mini review.
Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE
Cyanobacteria are an ancient group of photoautotrophic prokaryotes, and play an essential role in the global carbon cycle. They are also model organisms for studying photosynthesis and circadian regulation, and metabolic engineering and synthetic biology strategies grants light-driven biotechnological applications to cyanobacteria, especially for engineering cyanobacteria cells to achieve an efficient light-driven system for synthesizing any product of interest from renewable feedstocks. However, lower yield limits the potential of industrial application of cyanobacterial synthetic biology, and some key limitations must be overcome to realize the full biotechnological potential of these versatile microorganisms. Although genetic engineering toolkits for cyanobacteria have made some progress, the tools available still lag behind conventional heterotrophic microorganism. Consequently, this study describes the current situations and limitations of genetic engineering in cyanobacteria, and further improvements are proposed to improve the output of targeted products. We believe that cyanobacteria-mediated light-driven platforms towards efficient synthesis of green chemicals could unlock a bright future by developing the tools for strain manipulation and novel chassis organisms with excellent performance for biotechnological applications, which could also accelerate the advancement of bio-manufacturing industries.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Subject terms:
Biotechnology - Metabolic Engineering - Photosynthesis genetics - Synthetic Biology - Genetic Engineering - Cyanobacteria genetics - Cyanobacteria metabolismContent provider:
MEDLINE
Freely available online.
Recent advances in the application of genetic and epigenetic modalities in the improvement of antibody-producing cell lines.
Academic Journal
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Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE
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Baghini SS;Razeghian E;Malayer SK;Pecho RDC;Obaid M;Awfi ZS;Zainab HA;Shams...
Academic Journal
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Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE
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There are numerous applications for recombinant antibodies (rAbs) in biological and to...
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Recent advances in the application of genetic and epigenetic modalities in the improvement of antibody-producing cell lines.
Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE
There are numerous applications for recombinant antibodies (rAbs) in biological and toxicological research. Monoclonal antibodies are synthesized using genetic engineering and other related processes involved in the generation of rAbs. Because they can identify specific antigenic sites on practically any molecule, including medicines, hormones, microbial antigens, and cell receptors, rAbs are particularly useful in scientific research. The key benefits of rAbs are improved repeatability, control, and consistency, shorter manufacturing times than with hybridoma technology, an easier transition from one format of antibody to another, and an animal-free process. The engineering of the host cell has recently been developed method for enhancing the production efficiency and improving the quality of antibodies from mammalian cell lines. In this light, genetic engineering is mostly utilized to manage cellular chaperones, decrease cell death, increase cell viability, change the microRNAs (miRNAs) pattern in mammalian cells, and glycoengineered cell lines. Here, we shed light on how genetic engineering can be used therapeutically to produce antibodies at higher levels with greater potency and effectiveness.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Subject terms:
Animals - Recombinant Proteins genetics - Mammals - Antibody-Producing Cells - Epigenesis, Genetic - Genetic Engineering methods - Antibodies, Monoclonal geneticsContent provider:
MEDLINE
Plant chromosome engineering - past, present and future.
Academic Journal
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Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE
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Puchta H;Houben A
Academic Journal
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Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE
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Spontaneous chromosomal rearrangements (CRs) play an essential role in speciation, gen...
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Plant chromosome engineering - past, present and future.
Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE
Spontaneous chromosomal rearrangements (CRs) play an essential role in speciation, genome evolution and crop domestication. To be able to use the potential of CRs for breeding, plant chromosome engineering was initiated by fragmenting chromosomes by X-ray irradiation. With the rise of the CRISPR/Cas system, it became possible to induce double-strand breaks (DSBs) in a highly efficient manner at will at any chromosomal position. This has enabled a completely new level of predesigned chromosome engineering . The genetic linkage between specific genes can be broken by inducing chromosomal translocations. Natural inversions, which suppress genetic exchange, can be reverted for breeding. In addition, various approaches for constructing minichromosomes by downsizing regular standard A or supernumerary B chromosomes, which could serve as future vectors in plant biotechnology, have been developed. Recently, a functional synthetic centromere could be constructed. Also, different ways of genome haploidization have been set up, some based on centromere manipulations. In the future, we expect to see even more complex rearrangements, which can be combined with previously developed engineering technologies such as recombinases. Chromosome engineering might help to redefine genetic linkage groups, change the number of chromosomes, stack beneficial genes on mini cargo chromosomes, or set up genetic isolation to avoid outcrossing.
(© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.)
(© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.)
Subject terms:
Plant Breeding - Biotechnology - Centromere genetics - Chromosomes, Plant genetics - Genetic EngineeringContent provider:
MEDLINE
Beyond biotechnology : the barren promise of genetic engineering / Craig Holdrege and Steve Talbott.
Book
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2008
Book
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2008
Available at LC Collection (QH442 .H63 2008)
Comprehensive Screening of a Light-Inducible Split Cre Recombinase with Domain Insertion Profiling.
Academic Journal
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Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE
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Tague N;Andreani V;Fan Y;Timp W;Dunlop MJ
Academic Journal
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Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE
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Splitting proteins with light- or chemically inducible dimers provides a mechanism for...
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Comprehensive Screening of a Light-Inducible Split Cre Recombinase with Domain Insertion Profiling.
Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE
Splitting proteins with light- or chemically inducible dimers provides a mechanism for post-translational control of protein function. However, current methods for engineering stimulus-responsive split proteins often require significant protein engineering expertise and the laborious screening of individual constructs. To address this challenge, we use a pooled library approach that enables rapid generation and screening of nearly all possible split protein constructs in parallel, where results can be read out by using sequencing. We perform our method on Cre recombinase with optogenetic dimers as a proof of concept, resulting in comprehensive data on the split sites throughout the protein. To improve the accuracy in predicting split protein behavior, we develop a Bayesian computational approach to contextualize errors inherent to experimental procedures. Overall, our method provides a streamlined approach for achieving inducible post-translational control of a protein of interest.
Subject terms:
Bayes Theorem - Protein Engineering - Proteins - Genetic Engineering methods - Integrases genetics - Integrases metabolismContent provider:
MEDLINE
The case against perfection : ethics in the age of genetic engineering / Michael J. Sandel.
Book
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2007
Book
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2007
Available at Dewey Collection (174.957 Sa562c)
The Causes for Genomic Instability and How to Try and Reduce Them Through Rational Design of Synthetic DNA.
Academic Journal
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Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
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Arbel-Groissman M;Menuhin-Gruman I;Yehezkeli H;Naki D;Bergman S;Udi Y;Tulle...
Academic Journal
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Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
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Genetic engineering has revolutionized our ability to manipulate DNA and engineer orga...
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The Causes for Genomic Instability and How to Try and Reduce Them Through Rational Design of Synthetic DNA.
Genetic engineering has revolutionized our ability to manipulate DNA and engineer organisms for various applications. However, this approach can lead to genomic instability, which can result in unwanted effects such as toxicity, mutagenesis, and reduced productivity. To overcome these challenges, smart design of synthetic DNA has emerged as a promising solution. By taking into consideration the intricate relationships between gene expression and cellular metabolism, researchers can design synthetic constructs that minimize metabolic stress on the host cell, reduce mutagenesis, and increase protein yield. In this chapter, we summarize the main challenges of genomic instability in genetic engineering and address the dangers of unknowingly incorporating genomically unstable sequences in synthetic DNA. We also demonstrate the instability of those sequences by the fact that they are selected against conserved sequences in nature. We highlight the benefits of using ESO, a tool for the rational design of DNA for avoiding genetically unstable sequences, and also summarize the main principles and working parameters of the software that allow maximizing its benefits and impact.
(© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
(© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
