Phagocytes : biology, physiology, pathology, and pharmacotherapeutics / edited by Rodolfo Paoletti, Antonia Notario, and Giovanni Ricevuti.
Book
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1997
Available at Available Merrill-Cazier BARN, Books, Circulation Desk (1st Floor) (Call number: Q 11 .N5 V. 832)
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The CNS mononuclear phagocyte system in health and disease.
Academic Journal
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Publisher: Cell Press Country of Publication: United States NLM ID: 8809320 Publication Model: Print Cited Medium: Internet ISSN: 1097-4199 (Electronic) Linking ISSN: 08966273 NLM ISO Abbreviation: Neuron Subsets: MEDLINE
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Mundt S;Greter M;Becher B
Academic Journal
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Publisher: Cell Press Country of Publication: United States NLM ID: 8809320 Publication Model: Print Cited Medium: Internet ISSN: 1097-4199 (Electronic) Linking ISSN: 08966273 NLM ISO Abbreviation: Neuron Subsets: MEDLINE
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CNS-resident macrophages-including parenchymal microglia and border-associated macroph...
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The CNS mononuclear phagocyte system in health and disease.
Publisher: Cell Press Country of Publication: United States NLM ID: 8809320 Publication Model: Print Cited Medium: Internet ISSN: 1097-4199 (Electronic) Linking ISSN: 08966273 NLM ISO Abbreviation: Neuron Subsets: MEDLINE
CNS-resident macrophages-including parenchymal microglia and border-associated macrophages (BAMs)-contribute to neuronal development and health, vascularization, and tissue integrity at steady state. Border-patrolling mononuclear phagocytes such as dendritic cells and monocytes confer important immune functions to the CNS, protecting it from pathogenic threats including aberrant cell growth and brain malignancies. Even though we have learned much about the contribution of lymphocytes to CNS pathologies, a better understanding of differential roles of tissue-resident and -invading phagocytes is slowly emerging. In this perspective, we propose that in CNS neuroinflammatory diseases, tissue-resident macrophages (TRMs) contribute to the clearing of debris and resolution of inflammation, whereas blood-borne phagocytes are drivers of immunopathology. We discuss the remaining challenges to resolve which specialized mononuclear phagocyte populations are driving or suppressing immune effector function, thereby potentially dictating the outcome of autoimmunity or brain cancer.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Subject terms:
Humans - Microglia - Phagocytes pathology - Macrophages - Monocytes - Mononuclear Phagocyte System - Brain Neoplasms pathologyContent provider:
MEDLINE
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Methods for studying mononuclear phagocytes / edited by Dolph O. Adams, Paul J. Edelson, Hillel S. Koren.
Book
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1981
Available at Available Merrill-Cazier Books (3rd Floor North) (Call number: QP95.7 .M47)
ScienceDirect Available online: Click to view
Anaerobic respiration of host-derived methionine sulfoxide protects intracellular Salmonella from the phagocyte NADPH oxidase.
Academic Journal
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Publisher: Cell Press Country of Publication: United States NLM ID: 101302316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1934-6069 (Electronic) Linking ISSN: 19313128 NLM ISO Abbreviation: Cell Host Microbe Subsets: MEDLINE
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Kim JS;Liu L;Kant S;Orlicky DJ;Uppalapati S;Margolis A;Davenport BJ;Morriso...
Academic Journal
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Publisher: Cell Press Country of Publication: United States NLM ID: 101302316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1934-6069 (Electronic) Linking ISSN: 19313128 NLM ISO Abbreviation: Cell Host Microbe Subsets: MEDLINE
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Intracellular Salmonella experiencing oxidative stress downregulates aerobic respirati...
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Anaerobic respiration of host-derived methionine sulfoxide protects intracellular Salmonella from the phagocyte NADPH oxidase.
Publisher: Cell Press Country of Publication: United States NLM ID: 101302316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1934-6069 (Electronic) Linking ISSN: 19313128 NLM ISO Abbreviation: Cell Host Microbe Subsets: MEDLINE
Intracellular Salmonella experiencing oxidative stress downregulates aerobic respiration. To maintain cellular energetics during periods of oxidative stress, intracellular Salmonella must utilize terminal electron acceptors of lower energetic value than molecular oxygen. We show here that intracellular Salmonella undergoes anaerobic respiration during adaptation to the respiratory burst of the phagocyte NADPH oxidase in macrophages and in mice. Reactive oxygen species generated by phagocytes oxidize methionine, generating methionine sulfoxide. Anaerobic Salmonella uses the molybdenum cofactor-containing DmsABC enzymatic complex to reduce methionine sulfoxide. The enzymatic activity of the methionine sulfoxide reductase DmsABC helps Salmonella maintain an alkaline cytoplasm that supports the synthesis of the antioxidant hydrogen sulfide via cysteine desulfuration while providing a source of methionine and fostering redox balancing by associated dehydrogenases. Our investigations demonstrate that nontyphoidal Salmonella responding to oxidative stress exploits the anaerobic metabolism associated with dmsABC gene products, a pathway that has accrued inactivating mutations in human-adapted typhoidal serovars.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Subject terms:
Animals - Mice - Humans - Anaerobiosis - Methionine metabolism - Salmonella typhimurium metabolism - Respiration - NADPH Oxidases - Phagocytes metabolism - Methionine analogs & derivativesContent provider:
MEDLINE
Phagocytosis of bacteria and bacterial pathogenicity / edited by Joel D. Ernst and Olle Stendahl.
Book
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2006
Available at Available Merrill-Cazier Books (3rd Floor North) (Call number: QR 187 .P4 P489 2006)
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| Location | Call No. | Status |
|---|---|---|
| Merrill-Cazier Books (3rd Floor North) | QR 187 .P4 P489 2006 | Available |
Evaluation of murine renal phagocyte -fungal interactions using intravital confocal microscopy and flow cytometry.
Academic Journal
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Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
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Desai JV;Lionakis MS
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Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
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Myeloid phagocytes are essential for antifungal host defense during systemic candidias...
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Evaluation of murine renal phagocyte -fungal interactions using intravital confocal microscopy and flow cytometry.
Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
Myeloid phagocytes are essential for antifungal host defense during systemic candidiasis. Here, we present a protocol for assessing phagocyte -fungal interactions in vivo in the kidney, the primary target organ of the murine systemic candidiasis model. We describe steps for intravital confocal microscopy and flow cytometry. We also detail a kidney tissue dissociation procedure to obtain highly pure functional phagocytes for utilization in downstream ex vivo fungal uptake and killing assays.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Published by Elsevier Inc.)
Competing Interests: Declaration of interests The authors declare no competing interests.
(Published by Elsevier Inc.)
Subject terms:
Mice - Animals - Flow Cytometry - Microscopy, Confocal - Phagocytes microbiology - Kidney diagnostic imaging - CandidiasisContent provider:
MEDLINE
The Respiratory burst and its physiological significance / edited by Anthony J. Sbarra and Robert R. Strauss.
Book
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1988
Available at Available Merrill-Cazier Books (3rd Floor North) (Call number: QR 185.8 .P45 R47 1988)
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| Location | Call No. | Status |
|---|---|---|
| Merrill-Cazier Books (3rd Floor North) | QR 185.8 .P45 R47 1988 | Available |
Structure of human phagocyte NADPH oxidase in the activated state.
Academic Journal
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Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE
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Liu X;Shi Y;Liu R;Song K;Chen L
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Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE
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Phagocyte NADPH oxidase, a protein complex with a core made up of NOX2 and p22 subunit...
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Structure of human phagocyte NADPH oxidase in the activated state.
Phagocyte NADPH oxidase, a protein complex with a core made up of NOX2 and p22 subunits, is responsible for transferring electrons from intracellular NADPH to extracellular oxygen 1 . This process generates superoxide anions that are vital for killing pathogens 1 . The activation of phagocyte NADPH oxidase requires membrane translocation and the binding of several cytosolic factors 2 . However, the exact mechanism by which cytosolic factors bind to and activate NOX2 is not well understood. Here we present the structure of the human NOX2-p22 complex activated by fragments of three cytosolic factors: p47, p67 and Rac1. The structure reveals that the p67-Rac1 complex clamps onto the dehydrogenase domain of NOX2 and induces its contraction, which stabilizes the binding of NADPH and results in a reduction of the distance between the NADPH-binding domain and the flavin adenine dinucleotide (FAD)-binding domain. Furthermore, the dehydrogenase domain docks onto the bottom of the transmembrane domain of NOX2, which reduces the distance between FAD and the inner haem. These structural rearrangements might facilitate the efficient transfer of electrons between the redox centres in NOX2 and lead to the activation of phagocyte NADPH oxidase.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Subject terms:
Humans - Electrons - Enzyme Activation - Flavin-Adenine Dinucleotide metabolism - Heme chemistry - Heme metabolism - NADP metabolism - Protein Domains - Protein Subunits chemistry - Protein Subunits metabolism - Superoxides metabolism - Protein Binding - NADPH Oxidase 2 chemistry - NADPH Oxidase 2 metabolism - Phagocytes enzymologyContent provider:
MEDLINE
Phagocytic cells / editors, John I. Gallin, Anthony S. Fauci.
Book
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1982
Available at Available Merrill-Cazier Books (3rd Floor North) (Call number: QR 187 .P4 P485)
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| Location | Call No. | Status |
|---|---|---|
| Merrill-Cazier Books (3rd Floor North) | QR 187 .P4 P485 | Available |
Stretch-injury promotes microglia activation with enhanced phagocytic and synaptic stripping activities.
Academic Journal
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Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE
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Procès A;Alpizar YA;Halliez S;Brône B;Saudou F;Ris L;Gabriele S
Academic Journal
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Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE
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Microglial cells, as the primary defense line in the central nervous system, play a cr...
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Stretch-injury promotes microglia activation with enhanced phagocytic and synaptic stripping activities.
Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE
Microglial cells, as the primary defense line in the central nervous system, play a crucial role in responding to various mechanical signals that can trigger their activation. Despite extensive research on the impact of chemical signaling on brain cells, the understanding of mechanical signaling in microglia remains limited. To bridge this gap, we subjected microglial cells to a singular mechanical stretch and compared their responses with those induced by lipopolysaccharide treatment, a well-established chemical activator. Here we show that stretching microglial cells leads to their activation, highlighting their significant mechanosensitivity. Stretched microglial cells exhibited distinct features, including elevated levels of Iba1 protein, a denser actin cytoskeleton, and increased persistence in migration. Unlike LPS-treated microglial cells, the secretory profile of chemokines and cytokines remained largely unchanged in response to stretching, except for TNF-α. Intriguingly, a single stretch injury resulted in more compacted chromatin and DNA damage, suggesting potential long-term genomic instabilities in stretched microglia. Using compartmentalized microfluidic chambers with neuronal networks, we observed that stretched microglial cells exhibited enhanced phagocytic and synaptic stripping activities. These findings collectively suggest that stretching events can unlock the immune potential of microglial cells, contributing to the maintenance of brain tissue homeostasis following mechanical injury.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Sylvain Gabriele reports financial support was provided by University of Mons. Anthony Proces reports financial support was provided by Fund for Scientific Research. Yeranddy A. Alpizar reports financial support was provided by Research Foundation Flanders. Frederic Saudou reports financial support was provided by European Research Council.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Sylvain Gabriele reports financial support was provided by University of Mons. Anthony Proces reports financial support was provided by Fund for Scientific Research. Yeranddy A. Alpizar reports financial support was provided by Research Foundation Flanders. Frederic Saudou reports financial support was provided by European Research Council.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Subject terms:
Central Nervous System - Brain - Signal Transduction - Lipopolysaccharides pharmacology - Microglia metabolism - PhagocytesContent provider:
MEDLINE
Nonlinear current-voltage relationships in cultured macrophages / E.K. Gallin, D.R. Livengood.
Government Document
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1980
Available at Available Merrill-Cazier Government Documents (Lower Level) (Call number: D 15.12:80-8)
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| Location | Call No. | Status |
|---|---|---|
| Merrill-Cazier Government Documents (Lower Level) | D 15.12:80-8 | Available |
Functional In Vivo Imaging of Macrophages.
Academic Journal
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Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
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Wegner A;Palmisano R;Uderhardt S
Academic Journal
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Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
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Resident tissue macrophages (RTMs) are specialized phagocytes that are widely distribu...
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Functional In Vivo Imaging of Macrophages.
Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
Resident tissue macrophages (RTMs) are specialized phagocytes that are widely distributed throughout the body and are responsible for maintaining homeostasis. Recent advances in experimental techniques have enabled us to gain a greater insight into the actual in vivo biology of RTMs by observing their spatiotemporal dynamics directly in their native environment. Here, we detail a method for live tracking macrophages in a prototypical stromal tissue with high spatial and temporal resolution and great experimental versatility. Our approach builds on a custom intravital imaging platform and straightforward surgical preparation to gain access to an intact stromal compartment in order to analyze the morphological and behavioral dynamics of RTMs at single-cell resolution before and after experimental intervention. Furthermore, our versatile approach can also be utilized for live visualization of intracellular signaling and even for tracking cell organelles at subcellular resolution, and can be combined with downstream analyses such as multiplex confocal imaging, providing a unique insight into macrophage biology in vivo.
(© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
(© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Subject terms:
Humans - Diagnostic Imaging - Homeostasis - Preoperative Care - Macrophages - PhagocytesContent provider:
MEDLINE
Quantitative live-cell imaging of Candida albicans escape from immune phagocytes .
Academic Journal
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Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
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Olivier FAB;Traven A
Academic Journal
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Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
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Population-level dynamics of host-pathogen interactions can be characterized using qua...
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Quantitative live-cell imaging of Candida albicans escape from immune phagocytes .
Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
Population-level dynamics of host-pathogen interactions can be characterized using quantitative live-cell imaging. Here, we present a protocol for infecting macrophages with the fungal pathogen Candida albicans in vitro and quantitative live-cell imaging of immune and pathogen responses. We describe steps for detailed image analysis and provide resources for quantification of phagocytosis and pathogen escape, as well as macrophage membrane permeabilization and viability. This protocol is modifiable for applications with a range of pathogens, immune cell types, and host-pathogen mechanisms. For complete details on the use and execution of this protocol, please refer to Olivier et al. 1 .
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Subject terms:
Phagocytosis - Macrophages metabolism - Host-Pathogen Interactions - Candida albicans metabolism - PhagocytesContent provider:
MEDLINE
Effect of storage on the nitro blue tetrazolium reduction test in dog blood samples.
Academic Journal
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Publisher: American Society for Veterinary Clinical Pathology Country of Publication: United States NLM ID: 9880575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1939-165X (Electronic) Linking ISSN: 02756382 NLM ISO Abbreviation: Vet Clin Pathol Subsets: MEDLINE
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Baxarias M;Solano-Gallego L
Academic Journal
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Publisher: American Society for Veterinary Clinical Pathology Country of Publication: United States NLM ID: 9880575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1939-165X (Electronic) Linking ISSN: 02756382 NLM ISO Abbreviation: Vet Clin Pathol Subsets: MEDLINE
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Introduction: The nitro blue tetrazolium (NBT) reduction test (NBTT) has been used for...
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Effect of storage on the nitro blue tetrazolium reduction test in dog blood samples.
Publisher: American Society for Veterinary Clinical Pathology Country of Publication: United States NLM ID: 9880575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1939-165X (Electronic) Linking ISSN: 02756382 NLM ISO Abbreviation: Vet Clin Pathol Subsets: MEDLINE
Introduction: The nitro blue tetrazolium (NBT) reduction test (NBTT) has been used for measuring the metabolic activity of phagocytes of mammals. Activated neutrophils transform NBT into formazan in the cytoplasm. The NBTT can detect the activation of neutrophils in peripheral blood and is used to assess neutrophil function in dogs. However, the NBTT is not used frequently in the clinical setting, as samples should be processed after blood collection.
Objective: The aim of this study was to evaluate the effect of storage on NBTT in dog blood samples.
Materials and Methods: Residual EDTA blood samples from 22 dogs were included of different ages, breeds, and sex. The buffy coat layer was separated from the blood and incubated with 0.1% NBT. The NBTT was performed at 0, 24, 48, and 72 h after the collection of blood. Blood samples were stored at 4°C until the tests were performed. Blood smears were evaluated by light microscopy, and the NBT reduction rate was reported, which represents the percentage of activated neutrophils. The NBT reduction rate was calculated after counting 300 neutrophils in each slide.
Results: The means of NBTT in dog blood samples at 0, 24, 48, and 72 h were 8.3%, 8.5%, 8.7%, and 7.8%, respectively. No significant differences were observed between time points.
Conclusions: This study showed that the NBTT can be performed up to 72 h after the collection of canine blood if correctly refrigerated at 4°C. This finding supports the performance of the NBTT in the clinical setting.
(© 2023 The Authors. Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of American Society for Veterinary Clinical Pathology.)
Objective: The aim of this study was to evaluate the effect of storage on NBTT in dog blood samples.
Materials and Methods: Residual EDTA blood samples from 22 dogs were included of different ages, breeds, and sex. The buffy coat layer was separated from the blood and incubated with 0.1% NBT. The NBTT was performed at 0, 24, 48, and 72 h after the collection of blood. Blood samples were stored at 4°C until the tests were performed. Blood smears were evaluated by light microscopy, and the NBT reduction rate was reported, which represents the percentage of activated neutrophils. The NBT reduction rate was calculated after counting 300 neutrophils in each slide.
Results: The means of NBTT in dog blood samples at 0, 24, 48, and 72 h were 8.3%, 8.5%, 8.7%, and 7.8%, respectively. No significant differences were observed between time points.
Conclusions: This study showed that the NBTT can be performed up to 72 h after the collection of canine blood if correctly refrigerated at 4°C. This finding supports the performance of the NBTT in the clinical setting.
(© 2023 The Authors. Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of American Society for Veterinary Clinical Pathology.)
Subject terms:
Dogs - Animals - Nitroblue Tetrazolium metabolism - Nitroblue Tetrazolium pharmacology - Oxidation-Reduction - Leukocyte Count veterinary - Mammals metabolism - Neutrophils - PhagocytesContent provider:
MEDLINE
Liposomes - Human phagocytes interplay in whole blood: effect of liposome design.
Academic Journal
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Publisher: Elsevier Country of Publication: United States NLM ID: 101233142 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-9642 (Electronic) Linking ISSN: 15499634 NLM ISO Abbreviation: Nanomedicine Subsets: MEDLINE
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Giambelluca M;Markova E;Louet C;Steinkjer B;Sundset R;Škalko-Basnet N;Hak S
Academic Journal
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Publisher: Elsevier Country of Publication: United States NLM ID: 101233142 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-9642 (Electronic) Linking ISSN: 15499634 NLM ISO Abbreviation: Nanomedicine Subsets: MEDLINE
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Nanomedicine holds immense potential for therapeutic manipulation of phagocytic immune...
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Liposomes - Human phagocytes interplay in whole blood: effect of liposome design.
Publisher: Elsevier Country of Publication: United States NLM ID: 101233142 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-9642 (Electronic) Linking ISSN: 15499634 NLM ISO Abbreviation: Nanomedicine Subsets: MEDLINE
Nanomedicine holds immense potential for therapeutic manipulation of phagocytic immune cells. However, in vitro studies often fail to accurately translate to the complex in vivo environment. To address this gap, we employed an ex vivo human whole-blood assay to evaluate liposome interactions with immune cells. We systematically varied liposome size, PEG-surface densities and sphingomyelin and ganglioside content. We observed differential uptake patterns of the assessed liposomes by neutrophils and monocytes, emphasizing the importance of liposome design. Interestingly, our results aligned closely with published in vivo observations in mice and patients. Moreover, liposome exposure induced changes in cytokine release and cellular responses, highlighting the potential modulation of immune system. Our study highlights the utility of human whole-blood models in assessing nanoparticle-immune cell interactions and provides insights into liposome design for modulating immune responses.
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Subject terms:
Humans - Animals - Mice - Monocytes - Sphingomyelins - Cytokines - Liposomes - PhagocytesContent provider:
MEDLINE
Real-Time, In Situ Imaging of Macrophages via Phase-Change Peptide Nanoemulsions.
Academic Journal
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Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101235338 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1613-6829 (Electronic) Linking ISSN: 16136810 NLM ISO Abbreviation: Small Subsets: MEDLINE
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Kim I;Elliott JC;Lawanprasert A;Wood GM;Simon JC;Medina SH
Academic Journal
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Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101235338 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1613-6829 (Electronic) Linking ISSN: 16136810 NLM ISO Abbreviation: Small Subsets: MEDLINE
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Macrophages are specialized phagocytes that play central roles in immunity and tissue ...
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Real-Time, In Situ Imaging of Macrophages via Phase-Change Peptide Nanoemulsions.
Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101235338 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1613-6829 (Electronic) Linking ISSN: 16136810 NLM ISO Abbreviation: Small Subsets: MEDLINE
Macrophages are specialized phagocytes that play central roles in immunity and tissue repair. Their diverse functionalities have led to an evolution of new allogenic and autologous macrophage products. However, realizing the full therapeutic potential of these cell-based therapies requires development of imaging technologies that can track immune cell migration within tissues in real-time. Such innovations will not only inform treatment regimens and empower interpretation of therapeutic outcomes but also enable prediction and early intervention during adverse events. Here, phase-changing nanoemulsion contrast agents are reported that permit real-time, continuous, and high-fidelity ultrasound imaging of macrophages in situ. Using a de novo designed peptide emulsifier, liquid perfluorocarbon nanoemulsions are prepared and show that rational control over interfacial peptide assembly affords formulations with tunable acoustic sensitivity, macrophage internalization, and in cellulo stability. Imaging experiments demonstrate that emulsion-loaded macrophages can be readily visualized using standard diagnostic B-mode and Doppler ultrasound modalities. This allows on-demand and long-term tracking of macrophages within porcine coronary arteries, as an exemplary model. The results demonstrate that this platform is poised to open new opportunities for non-invasive, contrast-enhanced imaging of cell-based immunotherapies in tissues, while leveraging the low-cost, portable, and safe nature of diagnostic ultrasound.
(© 2023 The Authors. Small published by Wiley-VCH GmbH.)
(© 2023 The Authors. Small published by Wiley-VCH GmbH.)
Subject terms:
Animals - Swine - Ultrasonography - Peptides - Macrophages - PhagocytesContent provider:
MEDLINE
Megavoltage radiotherapy effects on organs of the reticuloendothelial system.
Academic Journal
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Publisher: Sociedade Brasileira Para O Desenvolvimento Da Pesquisa Em Cirurgia Curso De Pos-Graduacao Em Tecnica Operatoria E Cirurgia Experimental Escola Paulista De Medicina Country of Publication: Brazil NLM ID: 9103983 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-2674 (Electronic) Linking ISSN: 01028650 NLM ISO Abbreviation: Acta Cir Bras Subsets: MEDLINE
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Pereira F;Petroianu A;Geraldo JM;Pereira C
Academic Journal
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Publisher: Sociedade Brasileira Para O Desenvolvimento Da Pesquisa Em Cirurgia Curso De Pos-Graduacao Em Tecnica Operatoria E Cirurgia Experimental Escola Paulista De Medicina Country of Publication: Brazil NLM ID: 9103983 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-2674 (Electronic) Linking ISSN: 01028650 NLM ISO Abbreviation: Acta Cir Bras Subsets: MEDLINE
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Purpose: To study the uptake capacity of cells from the reticuloendothelial system aft...
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Megavoltage radiotherapy effects on organs of the reticuloendothelial system.
Publisher: Sociedade Brasileira Para O Desenvolvimento Da Pesquisa Em Cirurgia Curso De Pos-Graduacao Em Tecnica Operatoria E Cirurgia Experimental Escola Paulista De Medicina Country of Publication: Brazil NLM ID: 9103983 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-2674 (Electronic) Linking ISSN: 01028650 NLM ISO Abbreviation: Acta Cir Bras Subsets: MEDLINE
Purpose: To study the uptake capacity of cells from the reticuloendothelial system after irradiation with high-energy X-rays.
Methods: Eighteen male Wistar rats were distributed in three groups: group A (n = 6): control, unirradiated animals studied alongside animals from group B; group B (n = 6) and group C (n = 6): animals irradiated and studied after 24 and 48 hours, respectively. The rats were anesthetized and placed on a 10 MV linear accelerator. Next, they were irradiated in the abdominal region, with 8 Gy. Twenty-four (groups A and B) and 48 hours later (group C), a colloidal carbon solution (1 mL/kg) was intravenously injected in the tail vein. Fifty minutes later, the spleens and livers were withdrawn and prepared to be studied. Kupffer cells and splenic macrophages containing carbon pigments were counted in an optical microscope. Arithmetic means were calculated for each group and compared among them.
Results: X-rays were associated with a reduced number of Kupffer cells containing colloidal carbon, proliferation and enlargement of biliary ducts, hypoplasia, and hepatocyte necrosis. In the irradiated spleen, the colloidal carbon uptake was concentrated in the marginal zone around the white pulp, with an inexpressive uptake of pigments by macrophages from white and red pulps.
Conclusions: The X-rays in the rat abdomen are associated with a reduction in the Kupffer cells uptake of colloidal carbon, hepatocyte disorders, bile duct proliferation, and splenic uptake of colloidal carbon concentrated in the marginal zone.
Methods: Eighteen male Wistar rats were distributed in three groups: group A (n = 6): control, unirradiated animals studied alongside animals from group B; group B (n = 6) and group C (n = 6): animals irradiated and studied after 24 and 48 hours, respectively. The rats were anesthetized and placed on a 10 MV linear accelerator. Next, they were irradiated in the abdominal region, with 8 Gy. Twenty-four (groups A and B) and 48 hours later (group C), a colloidal carbon solution (1 mL/kg) was intravenously injected in the tail vein. Fifty minutes later, the spleens and livers were withdrawn and prepared to be studied. Kupffer cells and splenic macrophages containing carbon pigments were counted in an optical microscope. Arithmetic means were calculated for each group and compared among them.
Results: X-rays were associated with a reduced number of Kupffer cells containing colloidal carbon, proliferation and enlargement of biliary ducts, hypoplasia, and hepatocyte necrosis. In the irradiated spleen, the colloidal carbon uptake was concentrated in the marginal zone around the white pulp, with an inexpressive uptake of pigments by macrophages from white and red pulps.
Conclusions: The X-rays in the rat abdomen are associated with a reduction in the Kupffer cells uptake of colloidal carbon, hepatocyte disorders, bile duct proliferation, and splenic uptake of colloidal carbon concentrated in the marginal zone.
Subject terms:
Rats - Male - Animals - Rats, Wistar - Kupffer Cells - Liver - Carbon pharmacology - Mononuclear Phagocyte System - MacrophagesContent provider:
MEDLINE
Phagocytic clearance of dying cells and its implications.
Academic Journal
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Publisher: Blackwell Country of Publication: England NLM ID: 7702118 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-065X (Electronic) Linking ISSN: 01052896 NLM ISO Abbreviation: Immunol Rev Subsets: MEDLINE
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Ravichandran KS
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Publisher: Blackwell Country of Publication: England NLM ID: 7702118 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-065X (Electronic) Linking ISSN: 01052896 NLM ISO Abbreviation: Immunol Rev Subsets: MEDLINE
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Phagocytic clearance of dying cells and its implications.
Publisher: Blackwell Country of Publication: England NLM ID: 7702118 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-065X (Electronic) Linking ISSN: 01052896 NLM ISO Abbreviation: Immunol Rev Subsets: MEDLINE
Subject terms:
Humans - Apoptosis - Phagocytes - PhagocytosisContent provider:
MEDLINE
Pro-phagocytic function and structural basis of GPR84 signaling.
Academic Journal
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Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
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Zhang X;Wang Y;Supekar S;Cao X;Zhou J;Dang J;Chen S;Jenkins L;Marsango S;Li...
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Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
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GPR84 is a unique orphan G protein-coupled receptor (GPCR) that can be activated by en...
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Pro-phagocytic function and structural basis of GPR84 signaling.
Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
GPR84 is a unique orphan G protein-coupled receptor (GPCR) that can be activated by endogenous medium-chain fatty acids (MCFAs). The signaling of GPR84 is largely pro-inflammatory, which can augment inflammatory response, and GPR84 also functions as a pro-phagocytic receptor to enhance phagocytic activities of macrophages. In this study, we show that the activation of GPR84 by the synthetic agonist 6-OAU can synergize with the blockade of CD47 on cancer cells to induce phagocytosis of cancer cells by macrophages. We also determine a high-resolution structure of the GPR84-G i signaling complex with 6-OAU. This structure reveals an occluded binding pocket for 6-OAU, the molecular basis of receptor activation involving non-conserved structural motifs of GPR84, and an unusual G i -coupling interface. Together with computational docking and simulations studies, this structure also suggests a mechanism for the high selectivity of GPR84 for MCFAs and a potential routes of ligand binding and dissociation. These results provide a framework for understanding GPR84 signaling and developing new drugs targeting GPR84.
(© 2023. Springer Nature Limited.)
(© 2023. Springer Nature Limited.)
Subject terms:
Macrophages - Phagocytosis - Fatty Acids - Phagocytes - Signal TransductionContent provider:
MEDLINE
Fate mapping reveals compartment-specific clonal expansion of mononuclear phagocytes during kidney disease.
Report
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Publisher: Elsevier Country of Publication: United States NLM ID: 0323470 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1523-1755 (Electronic) Linking ISSN: 00852538 NLM ISO Abbreviation: Kidney Int Subsets: MEDLINE
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Yin J;Mei Q;Prinz M;Abdullah Z;Panzer U;Li J;von Vietinghoff S;Kurts C
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Publisher: Elsevier Country of Publication: United States NLM ID: 0323470 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1523-1755 (Electronic) Linking ISSN: 00852538 NLM ISO Abbreviation: Kidney Int Subsets: MEDLINE
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Fate mapping reveals compartment-specific clonal expansion of mononuclear phagocytes during kidney disease.
Publisher: Elsevier Country of Publication: United States NLM ID: 0323470 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1523-1755 (Electronic) Linking ISSN: 00852538 NLM ISO Abbreviation: Kidney Int Subsets: MEDLINE
Subject terms:
Humans - Macrophages - Monocytes - Phagocytes - Kidney DiseasesContent provider:
MEDLINE
In vivo imaging of the phagocytic dynamics underlying efficient clearance of adult-born hippocampal granule cells by ramified microglia.
Academic Journal
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Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8806785 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-1136 (Electronic) Linking ISSN: 08941491 NLM ISO Abbreviation: Glia Subsets: MEDLINE
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Kamei R;Okabe S
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Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8806785 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-1136 (Electronic) Linking ISSN: 08941491 NLM ISO Abbreviation: Glia Subsets: MEDLINE
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The phagocytosis of dead cells by microglia is essential in brain development and home...
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In vivo imaging of the phagocytic dynamics underlying efficient clearance of adult-born hippocampal granule cells by ramified microglia.
Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8806785 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-1136 (Electronic) Linking ISSN: 08941491 NLM ISO Abbreviation: Glia Subsets: MEDLINE
The phagocytosis of dead cells by microglia is essential in brain development and homeostasis. However, the mechanism underlying the efficient removal of cell corpses by ramified microglia remains poorly understood. Here, we investigated the phagocytosis of dead cells by ramified microglia in the hippocampal dentate gyrus, where adult neurogenesis and homeostatic cell clearance occur. Two-color imaging of microglia and apoptotic newborn neurons revealed two important characteristics. Firstly, frequent environmental surveillance and rapid engulfment reduced the time required for dead cell clearance. The motile microglial processes frequently contacted and enwrapped apoptotic neurons at the protrusion tips and completely digested them within 3-6 h of the initial contact. Secondly, while a single microglial process engaged in phagocytosis, the remaining processes continued environmental surveillance and initiated the removal of other dead cells. The simultaneous removal of multiple dead cells increases the clearance capacity of a single microglial cell. These two characteristics of ramified microglia contributed to their phagocytic speed and capacity, respectively. Consistently, the cell clearance rate was estimated to be 8-20 dead cells/microglia/day, supporting the efficiency of removing apoptotic newborn neurons. We concluded that ramified microglia specialize in utilizing individual motile processes to detect stochastic cell death events and execute parallel phagocytoses.
(© 2023 The Authors. GLIA published by Wiley Periodicals LLC.)
(© 2023 The Authors. GLIA published by Wiley Periodicals LLC.)
Subject terms:
Adult - Humans - Infant, Newborn - Hippocampus metabolism - Phagocytosis physiology - Neurons metabolism - Brain - Microglia metabolism - PhagocytesContent provider:
MEDLINE
Leishmania major drives host phagocyte death and cell-to-cell transfer depending on intracellular pathogen proliferation rate.
Academic Journal
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Publisher: American Society for Clinical Investigation Country of Publication: United States NLM ID: 101676073 Publication Model: Electronic Cited Medium: Internet ISSN: 2379-3708 (Electronic) Linking ISSN: 23793708 NLM ISO Abbreviation: JCI Insight Subsets: MEDLINE
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Baars I;Jaedtka M;Dewitz LA;Fu Y;Franz T;Mohr J;Gintschel P;Berlin H;Degen ...
Academic Journal
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Publisher: American Society for Clinical Investigation Country of Publication: United States NLM ID: 101676073 Publication Model: Electronic Cited Medium: Internet ISSN: 2379-3708 (Electronic) Linking ISSN: 23793708 NLM ISO Abbreviation: JCI Insight Subsets: MEDLINE
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The virulence of intracellular pathogens relies largely on the ability to survive and ...
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Leishmania major drives host phagocyte death and cell-to-cell transfer depending on intracellular pathogen proliferation rate.
Publisher: American Society for Clinical Investigation Country of Publication: United States NLM ID: 101676073 Publication Model: Electronic Cited Medium: Internet ISSN: 2379-3708 (Electronic) Linking ISSN: 23793708 NLM ISO Abbreviation: JCI Insight Subsets: MEDLINE
The virulence of intracellular pathogens relies largely on the ability to survive and replicate within phagocytes but also on release and transfer into new host cells. Such cell-to-cell transfer could represent a target for counteracting microbial pathogenesis. However, our understanding of the underlying cellular and molecular processes remains woefully insufficient. Using intravital 2-photon microscopy of caspase-3 activation in the Leishmania major-infected (L. major-infected) live skin, we showed increased apoptosis in cells infected by the parasite. Also, transfer of the parasite to new host cells occurred directly without a detectable extracellular state and was associated with concomitant uptake of cellular material from the original host cell. These in vivo findings were fully recapitulated in infections of isolated human phagocytes . Furthermore, we observed that high pathogen proliferation increased cell death in infected cells, and long-term residency within an infected host cell was only possible for slowly proliferating parasites. Our results therefore suggest that L. major drives its own dissemination to new phagocytes by inducing host cell death in a proliferation-dependent manner.
Subject terms:
Humans - Virulence - Mice, Inbred C57BL - Cells, Cultured - Mice - Animals - Leishmania major pathogenicity - Phagocytes parasitology - ApoptosisContent provider:
MEDLINE
Single Cell Analysis of High-Parameter Histology Images Using Histoflow Cytometry.
Academic Journal
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Publisher: American Association of Immunologists Country of Publication: United States NLM ID: 2985117R Publication Model: Print Cited Medium: Internet ISSN: 1550-6606 (Electronic) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE
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Jain RW;Elliott DA;Yong VW
Academic Journal
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Publisher: American Association of Immunologists Country of Publication: United States NLM ID: 2985117R Publication Model: Print Cited Medium: Internet ISSN: 1550-6606 (Electronic) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE
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Immunofluorescence histology is commonly used to study immune cells in tissues where t...
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Single Cell Analysis of High-Parameter Histology Images Using Histoflow Cytometry.
Publisher: American Association of Immunologists Country of Publication: United States NLM ID: 2985117R Publication Model: Print Cited Medium: Internet ISSN: 1550-6606 (Electronic) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE
Immunofluorescence histology is commonly used to study immune cells in tissues where the number of fluorescence parameters is normally limited to four or less. This makes it impossible to interrogate multiple subsets of immune cells in tissue with the same precision as flow cytometry. The latter, however, dissociates tissues and loses spatial information. To bridge the gap between these technologies, we developed a workflow to expand the number of fluorescence parameters that can be imaged on widely available microscopes. We instituted a method for identifying single cells in tissue and exporting the data for flow cytometry-based analysis. This histoflow cytometry technique successfully separates spectrally overlapping dyes and identifies similar numbers of cells in tissue sections as manual cell counts. Populations identified through flow cytometry-like gating strategies are mapped to the original tissue to spatially localize gated subsets. We applied histoflow cytometry to immune cells in the spinal cords of mice with experimental autoimmune encephalomyelitis. We ascertained that B cells, T cells, neutrophils, and phagocytes differed in their frequencies in CNS immune cell infiltrates and were increased relative to healthy controls. Spatial analysis determined that B cells and T cells/phagocytes preferentially localized to CNS barriers and parenchyma, respectively. By spatially mapping these immune cells, we inferred their preferred interacting partners within immune cell clusters. Overall, we demonstrate the ease and utility of histoflow cytometry, which expands the number of fluorescent channels used in conventional immunofluorescence and enables quantitative cytometry and spatial localization of histological analyses.
(Copyright © 2023 by The American Association of Immunologists, Inc.)
(Copyright © 2023 by The American Association of Immunologists, Inc.)
